Direct identification of mycobacteria from smear-positive samples using real-time polymerase chain reaction

被引：3
|
作者：
Marekovic, I. [1 ,2 ]
Bosnjak, Z. [2 ]
Plecko, V. [1 ,2 ]
机构：
[1] Univ Zagreb, Sch Med, Zagreb 41001, Croatia
[2] Univ Hosp Ctr Zagreb, Dept Clin & Mol Microbiol, Zagreb 10000, Croatia
来源：
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2014年 / 18卷 / 08期
关键词：
acid-fast bacilli grade; bronchoalveolar lavage; tuberculosis; SPUTUM SAMPLES; PCR; TUBERCULOSIS;
D O I：
10.5588/ijtld.13.0042
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The aim of the present study was to investigate whether real-time polymerase chain reaction (PCR) has a low identification rate for samples with low acid-fast bacilli (AFB) grades, including those obtained using bronchoscopy. When 50 smear-positive samples were compared by AFB score and PCR result, PCR was 100% successful in identifying AFB 2+ and AFB 3+ samples. However, only 14 of 26(52%) AFB 1+ samples were identified. In paucibacillary smear-positive samples, PCR is not reliable enough to exclude tuberculosis, but in smear-positive patients with high AFB grades PCR can immediately change the clinical management of the disease.
引用
收藏
页码：978 / 980
页数：3
相关论文
共 50 条
  • [21] Quantitative detection of Clostridium difficile in hospital environmental samples by real-time polymerase chain reaction
    Mutters, R.
    Nonnenmacher, C.
    Susin, C.
    Albrecht, U.
    Kropatsch, R.
    Schumacher, S.
    JOURNAL OF HOSPITAL INFECTION, 2009, 71 (01) : 43 - 48
  • [22] Detection of toxin genes from Clostridium difficile by using real-time polymerase chain reaction
    Sun, Wen-Xuan
    Lu, Xiao-Juan
    Tian, Peng-Fei
    Zhang, Xiao-Fei
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2017, 10 (04): : 7049 - 7055
  • [23] Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis
    Babafemi, Emmanuel O.
    Cherian, Benny P.
    Banting, Lee
    Mills, Graham A.
    Ngianga, Kandala, II
    SYSTEMATIC REVIEWS, 2017, 6
  • [24] Detection of Mycobacterium Tuberculosis DNA from peripheral blood in patients with HIV-seronegative and new cases of smear-positive pulmonary tuberculosis by polymerase chain reaction
    Taci, N
    Yurdakul, AS
    Ceyhan, I
    Berktas, MB
    Ögretensoy, M
    RESPIRATORY MEDICINE, 2003, 97 (06) : 676 - 681
  • [25] Clinical usefulness of Mycobacterium tuberculosis/NTM real-time polymerase chain reaction in diagnosis of tuberculosis
    Rashad, Alaa
    Rashed, Hebat-Allah G.
    Zakaria, Mohammed
    Omar, Asmaa
    Nafady-Hego, Hanaa
    Hosni, Amal
    EGYPTIAN JOURNAL OF CHEST DISEASES AND TUBERCULOSIS, 2021, 70 (01): : 43 - 47
  • [26] Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit
    Yanagihara, Katsunori
    Kitagawa, Yuko
    Tomonaga, Masao
    Tsukasaki, Kunihiro
    Kohno, Shigeru
    Seki, Masafumi
    Sugimoto, Hisashi
    Shimazu, Takeshi
    Tasaki, Osamu
    Matsushima, Asako
    Ikeda, Yasuo
    Okamoto, Shinichiro
    Aikawa, Naoki
    Hori, Shingo
    Obara, Hideaki
    Ishizaka, Akitoshi
    Hasegawa, Naoki
    Takeda, Junzo
    Kamihira, Shimeru
    Sugahara, Kazuyuki
    Asari, Seishi
    Murata, Mitsuru
    Kobayashi, Yoshio
    Ginba, Hiroyuki
    Sumiyama, Yoshinobu
    Kitajima, Masaki
    CRITICAL CARE, 2010, 14 (04)
  • [27] Identification of chickpea (Cicer arietinum) in foods using a novel real-time polymerase chain reaction detection method
    Nakamura, Kosuke
    Ishigaki, Takumi
    Kobayashi, Tomoko
    Kimata, Shinya
    Soga, Keisuke
    Fujii, Uki
    Kishine, Masahiro
    Takabatake, Reona
    Mano, Junichi
    Kitta, Kazumi
    Kawakami, Hiroshi
    Nishimaki-Mogami, Tomoko
    Kondo, Kazunari
    JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2018, 71 : 8 - 16
  • [28] Direct detection and identification of acid-fast bacteria from smear-positive broth cultures using a pyrosequencing method
    Bao, Jian R.
    Clark, Richard B.
    Master, Ronald N.
    Piscitelli, Arelis E.
    Tummala, Praveena R.
    Eklund, Lynn L.
    Poselero, Beatriz G.
    Wright, Jackie
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2014, 79 (02) : 228 - 232
  • [29] Recent developments in polymerase chain reaction - Real-time PCR
    Stadejek, T
    MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE, 2006, 62 (04): : 390 - 394
  • [30] Assessment of Real-Time Polymerase Chain Reaction for the Detection of Trichostrongylus spp. DNA from Human Fecal Samples
    Perandin, Francesca
    Pomari, Elena
    Bonizzi, Camilla
    Mistretta, Manuela
    Formenti, Fabio
    Bisoffi, Zeno
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2018, 98 (03) : 768 - 771
12345