Direct identification of mycobacteria from smear-positive samples using real-time polymerase chain reaction

被引：3

作者：

Marekovic, I. ^{[1
,2
]}

Bosnjak, Z. ^{[2
]}

Plecko, V. ^{[1
,2
]}

机构：

[1] Univ Zagreb, Sch Med, Zagreb 41001, Croatia

[2] Univ Hosp Ctr Zagreb, Dept Clin & Mol Microbiol, Zagreb 10000, Croatia

来源：

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2014年 / 18卷 / 08期

关键词：

acid-fast bacilli grade; bronchoalveolar lavage; tuberculosis; SPUTUM SAMPLES; PCR; TUBERCULOSIS;

D O I：

10.5588/ijtld.13.0042

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of the present study was to investigate whether real-time polymerase chain reaction (PCR) has a low identification rate for samples with low acid-fast bacilli (AFB) grades, including those obtained using bronchoscopy. When 50 smear-positive samples were compared by AFB score and PCR result, PCR was 100% successful in identifying AFB 2+ and AFB 3+ samples. However, only 14 of 26(52%) AFB 1+ samples were identified. In paucibacillary smear-positive samples, PCR is not reliable enough to exclude tuberculosis, but in smear-positive patients with high AFB grades PCR can immediately change the clinical management of the disease.

引用

页码：978 / 980

页数：3

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