Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type I polyketide synthase gene

被引：12

作者：

Komaki, Hisayuki ^{[1
,2
]}

Izumikawa, Miho ^{[1
]}

Ueda, Jun-ya ^{[1
]}

Nakashima, Takuji ^{[2
]}

Khan, Shams Tabrez ^{[1
,2
]}

Takagi, Motoki ^{[1
]}

Shin-ya, Kazuo ^{[3
]}

机构：

[1] JBIC, BIRC, Koto Ku, Tokyo 1350064, Japan

[2] NITE, NBRC, Chiba 2920818, Japan

[3] Natl Inst Adv Ind Sci & Technol, BIRC, Koto Ku, Tokyo 1350064, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2009年 / 83卷 / 01期

关键词：

Type I polyketide synthase; Streptomyces bicolor; Polyene macrolide; JBIR-13; Pimaricin; COMPLETE GENOME SEQUENCE; STEREOSTRUCTURE; BIOSYNTHESIS; SPECTROSCOPY; ANTIBIOTICS; METABOLITES; PATHWAYS; MODEL;

D O I：

10.1007/s00253-008-1849-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746(T) revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746(T) was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.

引用

页码：127 / 133

页数：7

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