Cationic Nucleoside Lipids Based on a 3-Nitropyrrole Universal Base for siRNA Delivery

被引：32
|
作者：
Ceballos, Claire [3 ]
Prata, Carla A. H. [1 ,2 ]
Giorgio, Suzanne [3 ]
Garzino, Frederic
Payet, Dominique [4 ]
Barthelemy, Philippe [5 ]
Grinstaff, Mark W. [1 ,2 ]
Camplo, Michel [1 ,2 ,3 ]
机构：
[1] Boston Univ, Metcalf Ctr Sci & Engn, Dept Chem, Boston, MA 02215 USA
[2] Boston Univ, Metcalf Ctr Sci & Engn, Dept Biomed Engn, Boston, MA 02215 USA
[3] Univ Aix Marseille 2, CNRS, UPR 3118, Ctr Interdisciplinaire Nanosci Marseille CINaM, F-13288 Marseille 09, France
[4] Ctr Immunol Marseille Luminy, F-13288 Marseille 9, France
[5] Univ Bordeaux 2, INSERM, U869, F-33076 Bordeaux, France
来源：
BIOCONJUGATE CHEMISTRY | 2009年 / 20卷 / 02期
关键词：
SUPRAMOLECULAR ASSEMBLIES; GENE DELIVERY; DNA; NUCLEOTIDES; AMPHIPHILE; SYSTEMS;
D O I：
10.1021/bc800432n
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cationic nucleoside lipids based on a 3-nitropyrrole universal base were prepared from D-ribose using a straightforward chemical synthesis. Several studies including DLS, TEM, and ethidium bromide (EthBr) assay demonstrated that these amphiphilic molecules form supramolecular organizations of nanometer size in aqueous solutions and are able to bind nucleic acids. siRNA knockdown experiments were performed with these nucleolipids, and we observed protein knockdown activity similar to the siPORT NeoFX positive control. No significant cytotoxicity was found.
引用
收藏
页码：193 / 196
页数：4
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