Isolation and identification of a new hypocrellin A-producing strain Shiraia sp SUPER-H168

被引：49

作者：

Liang, Xiao-Hui ^{[1
]}

Cai, Yu-Jie ^{[1
]}

Liao, Xiang-Ru ^{[1
]}

Wu, Kang ^{[1
]}

Wang, Lei ^{[1
]}

Zhang, Da-Bing ^{[2
]}

Meng, Qiang ^{[2
]}

机构：

[1] Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Jiangsu 214122, Peoples R China

[2] Hanbon Sci & Technol Co Ltd, Huaian 223001, Peoples R China

来源：

MICROBIOLOGICAL RESEARCH | 2009年 / 164卷 / 01期

关键词：

Hypocrellin A; Production; Identification; Shiraia sp; 18S rDNA; PERYLENEQUINONES;

D O I：

10.1016/j.micres.2008.08.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new hypocrellin A-producing strain, Shiraia sp. SUPER-H168, was isolated from tissues of bamboo, Brochystachyum densiflorum. The morphology of this strain was characterized with a tight microscope and a scanning electronic microscope. The mycelia, conidia, pycnidia of fungus were observed. Small pycnidia (10-20 mu m in length) full of small conidia appeared on the mycelia, which were first reported in this study. The 18S rDNA region of this strain was amplified and sequenced. Then a neighbor-joining tree of 18S rDNA was constructed. According to the result of analysis, the strain SUPER-H168 was proved to belong to the genus Shiraia. Hypocrellin A was produced by solid-state fermentation, extracted by acetone, isolated by preparative RP-HPLC, and identified by RP-HPLC, ESI-MS and ultraviolet-visible absorbing scanning with diode array detection. The HA production could reach 2.02 mg/g dry solid substrate. (C) 2008 Elsevier GmbH. All rights reserved.

引用

页码：9 / 17

页数：9

共 24 条

[11]

[李达旭 Li Daxu], 2003, [四川大学学报. 自然科学版, Journal of Sichuan University.Natural Science edition], V40, P139

[12] HYPOCRELLIN-A SENSITIZED PHOTOOXIDATION OF BILIRUBIN [J].

MA, JS ;

YAN, F ;

WANG, CQ ;

AN, JY .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1989, 50 (06) :827-830

[13] Photodynamic inhibitory effects of three perylenequinones on human colorectal carcinoma cell line and primate embryonic stem cell line [J].

Ma, L ;

Tai, H ;

Li, C ;

Zhang, Y ;

Wang, ZH ;

Ji, WZ .

WORLD JOURNAL OF GASTROENTEROLOGY, 2003, 9 (03) :485-490

[14] STRUCTURE OF ELSINOCHROME-A - A PERYLENEQUINONE METABOLITE [J].

MEILLE, SV ;

MALPEZZI, L ;

ALLEGRA, G ;

NASINI, G ;

WEISS, U .

ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE COMMUNICATIONS, 1989, 45 :628-632

[15] Preclinical assessment of hypocrellin B and hypocrellin B derivatives as sensitizers for photodynamic therapy of cancer: Progress update [J].

Miller, GG ;

Brown, K ;

Ballangrud, AM ;

Barajas, O ;

Xiao, Z ;

Tulip, J ;

Lown, JW ;

Leithoff, JM ;

AllalunisTurner, MJ ;

Mehta, RD ;

Moore, RB .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 65 (04) :714-722

[16] Molecular diversity of bamboo-associated fungi isolated from Japan [J].

Morakotkarn, Doungporn ;

Kawasaki, Hiroko ;

Seki, Tatsuji .

FEMS MICROBIOLOGY LETTERS, 2007, 266 (01) :10-19

[17] RAPD-PCR, isozyme, rDNA RFLP and rDNA sequence analyses in identification of Finnish Fusarium oxysporum isolates [J].

Paavanen-Huhtala, S ;

Hyvönen, J ;

Bulat, SA ;

Yli-Mattila, T .

MYCOLOGICAL RESEARCH, 1999, 103 :625-634

[18] ANTHRAQUINONES AS A NEW CLASS OF ANTIVIRAL AGENTS AGAINST HUMAN-IMMUNODEFICIENCY-VIRUS [J].

SCHINAZI, RF ;

CHU, CK ;

BABU, JR ;

OSWALD, BJ ;

SAALMANN, V ;

CANNON, DL ;

ERIKSSON, BFH ;

NASR, M .

ANTIVIRAL RESEARCH, 1990, 13 (05) :265-272

[19] The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J].

Thompson, JD ;

Gibson, TJ ;

Plewniak, F ;

Jeanmougin, F ;

Higgins, DG .

NUCLEIC ACIDS RESEARCH, 1997, 25 (24) :4876-4882

[20]

WAN XY, 1981, KEXUE TONGBAO, V26, P1040

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