Isolation and identification of a new hypocrellin A-producing strain Shiraia sp SUPER-H168

被引:49
作者
Liang, Xiao-Hui [1 ]
Cai, Yu-Jie [1 ]
Liao, Xiang-Ru [1 ]
Wu, Kang [1 ]
Wang, Lei [1 ]
Zhang, Da-Bing [2 ]
Meng, Qiang [2 ]
机构
[1] Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Jiangsu 214122, Peoples R China
[2] Hanbon Sci & Technol Co Ltd, Huaian 223001, Peoples R China
关键词
Hypocrellin A; Production; Identification; Shiraia sp; 18S rDNA; PERYLENEQUINONES;
D O I
10.1016/j.micres.2008.08.004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new hypocrellin A-producing strain, Shiraia sp. SUPER-H168, was isolated from tissues of bamboo, Brochystachyum densiflorum. The morphology of this strain was characterized with a tight microscope and a scanning electronic microscope. The mycelia, conidia, pycnidia of fungus were observed. Small pycnidia (10-20 mu m in length) full of small conidia appeared on the mycelia, which were first reported in this study. The 18S rDNA region of this strain was amplified and sequenced. Then a neighbor-joining tree of 18S rDNA was constructed. According to the result of analysis, the strain SUPER-H168 was proved to belong to the genus Shiraia. Hypocrellin A was produced by solid-state fermentation, extracted by acetone, isolated by preparative RP-HPLC, and identified by RP-HPLC, ESI-MS and ultraviolet-visible absorbing scanning with diode array detection. The HA production could reach 2.02 mg/g dry solid substrate. (C) 2008 Elsevier GmbH. All rights reserved.
引用
收藏
页码:9 / 17
页数:9
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