Evaluating the reintroduction project of Przewalski's horse in China using genetic and pedigree data

被引:27
作者
Liu, Gang [1 ]
Shafer, Aaron B. A. [2 ]
Zimmermann, Waltraut [3 ]
Hu, Defu [1 ]
Wang, Wenting [1 ]
Chu, Hongjun [4 ]
Cao, Jie [5 ]
Zhao, Chongxue [6 ]
机构
[1] Beijing Forestry Univ, Coll Nat Conservat, Lab Noninvas Res Technol Endangered Species, Beijing, Peoples R China
[2] Uppsala Univ, Evolutionary Biol Ctr, Uppsala, Sweden
[3] Cologne Zool Garden, Cologne, Germany
[4] Forestry Bur Altay Prefecture, Altay, Peoples R China
[5] Wild Horse Breeding Ctr, Urumqi, Peoples R China
[6] Gansu Endangered Species Res Ctr, Wuwei, Peoples R China
关键词
Przewalski's horse; Microsatellite; Pedigree; Genetic diversity; Population structure; Reintroduction; CAPTIVE BREEDING PROGRAMS; ASSESS MANAGEMENT OPTIONS; MICROSATELLITE DNA; EQUUS-PRZEWALSKII; INBREEDING DEPRESSION; CONSERVATION GENETICS; COMPUTER-PROGRAM; GENOME SEQUENCE; RARE ALLELES; POPULATION;
D O I
10.1016/j.biocon.2013.11.022
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Przewalski's horse went extinct in the wild in the mid 1960s. Starting in 1985, individuals were brought from western zoos to two centers in China and breeding programs were initiated. With the increasing size of captive populations, two reintroduction projects were launched in the northwestern China in 2001 and 2010. Knowledge on genetic diversity in China's horse populations is limited, but would help improve the genetic management and assess the success of the reintroduction. Accordingly, one reintroduced and two captive populations were examined with 10 microsatellite loci together with pedigree data. The results showed higher level of diversity within the captive populations than the reintroduced population, indicating some alleles may have been lost during reintroduction. Genetic differentiation was detected among populations (F-ST = 0.09 +/- 0.05, Rhos(ST) = 0.05 +/- 0.02) and Bayesian clustering supported the presence of three subpopulations. The highest genetic differentiation was observed between the captive and reintroduced populations, and inbreeding coefficients were generally higher in the reintroduced population. Temporal estimates of both pedigree and microsatellite data showed a high, but decreasing level inbreeding. Through simulations, we estimated that the reintroduced population needs more than 100 individuals to retain approximately 90% of its current, already depauperate, genetic diversity. We have provided recommendations for the management program concerning introgressed genes from domestic horse and the number and origin of individuals for future reintroductions. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:288 / 298
页数:11
相关论文
共 83 条
[1]   Genetic diversity in German draught horse breeds compared with a group of primitive, riding and wild horses by means of microsatellite DNA markers [J].
Aberle, KS ;
Hamann, H ;
Drögemüller, C ;
Distl, O .
ANIMAL GENETICS, 2004, 35 (04) :270-277
[2]   The influence of parental relatedness on reproductive success [J].
Amos, W ;
Wilmer, JW ;
Fullard, K ;
Burg, TM ;
Croxall, JP ;
Bloch, D ;
Coulson, T .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2001, 268 (1480) :2021-2027
[3]  
[Anonymous], 2010, CONSERV BIOL
[4]  
[Anonymous], 1999, POPGENE (Version 1.3. 1). Microsoft Window-Bases Freeware for Population Genetic Analysis
[5]   Genetic effects of captive breeding cause a rapid, cumulative fitness decline in the wild [J].
Araki, Hitoshi ;
Cooper, Becky ;
Blouin, Michael S. .
SCIENCE, 2007, 318 (5847) :100-103
[6]   Inbreeding depression in benign and stressful environments [J].
Armbruster, P ;
Reed, DH .
HEREDITY, 2005, 95 (03) :235-242
[7]   Directions in reintroduction biology [J].
Armstrong, Doug P. ;
Seddon, Philip J. .
TRENDS IN ECOLOGY & EVOLUTION, 2008, 23 (01) :20-25
[8]  
Ballou J.D., 2011, PMX SOFTWARE DEMOGRA
[9]  
Ballou Jonathan D., 1995, P76
[10]   Does heterozygosity estimate inbreeding in real populations? [J].
Balloux, F ;
Amos, W ;
Coulson, T .
MOLECULAR ECOLOGY, 2004, 13 (10) :3021-3031