A continuous-flow high-throughput microfluidic device for airborne bacteria PCR detection

被引：51

作者：

Jiang, Xiran ^{[1
]}

Jing, Wenwen ^{[1
,2
]}

Zheng, Lulu ^{[1
]}

Liu, Sixiu ^{[1
]}

Wu, Wenjuan ^{[3
]}

Sui, Guodong ^{[1
]}

机构：

[1] Fudan Univ, Inst Biomed Sci, Dept Environm Sci & Engn, Shanghai Key Lab Atmospher Particle Pollut Preven, Shanghai 200433, Peoples R China

[2] Fudan Univ, Sch Basic Med Sci, Dept Med Microbiol & Parasitol, Shanghai 200032, Peoples R China

[3] Tongji Univ, Shanghai East Hosp, Dept Lab Med, Shanghai 200120, Peoples R China

来源：

LAB ON A CHIP | 2014年 / 14卷 / 04期

关键词：

NUCLEIC-ACID AMPLIFICATION;

D O I：

10.1039/c3lc50977j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid analysis of airborne pathogens plays a critical role in early warning of spreading infectious diseases, which is essential for public health and disease prevention. Herein we report an integrated microfluidic device that can perform airborne pathogen capture, enrichment and continuous-flow high-throughput gene analysis. The device was validated by six frequently encountered bacteria and shows great potential in environmental analysis and for public health protection.

引用

页码：671 / 676

页数：6

共 23 条

[1]

Babu L., 2013, ANN MICROBIOL, V1, P1

[2] DETECTION OF ESCHERICHIA-COLI AND SHIGELLA SPP IN WATER BY USING THE POLYMERASE CHAIN-REACTION AND GENE PROBES FOR UID [J].

BEJ, AK ;

DICESARE, JL ;

HAFF, L ;

ATLAS, RM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (04) :1013-1017

[3] Nucleic acid amplification using microfluidic systems [J].

Chang, Chen-Min ;

Chang, Wen-Hsin ;

Wang, Chih-Hung ;

Wang, Jung-Hao ;

Mai, John D. ;

Lee, Gwo-Bin .

LAB ON A CHIP, 2013, 13 (07) :1225-1242

[4]

Changchun L., 2012, MICROFLUID NANOFLUID, V4, P923

[5] Continuous-flow thermal gradient PCR [J].

Crews, Niel ;

Wittwer, Carl ;

Gale, Bruce .

BIOMEDICAL MICRODEVICES, 2008, 10 (02) :187-195

[6] DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae [J].

Heidelberg, JF ;

Eisen, JA ;

Nelson, WC ;

Clayton, RA ;

Gwinn, ML ;

Dodson, RJ ;

Haft, DH ;

Hickey, EK ;

Peterson, JD ;

Umayam, L ;

Gill, SR ;

Nelson, KE ;

Read, TD ;

Tettelin, H ;

Richardson, D ;

Ermolaeva, MD ;

Vamathevan, J ;

Bass, S ;

Qin, HY ;

Dragoi, I ;

Sellers, P ;

McDonald, L ;

Utterback, T ;

Fleishmann, RD ;

Nierman, WC ;

White, O ;

Salzberg, SL ;

Smith, HO ;

Colwell, RR ;

Mekalanos, JJ ;

Venter, JC ;

Fraser, CM .

NATURE, 2000, 406 (6795) :477-483

[7] Use of a genus- and species-specific multiplex PCR for identification of enterococci [J].

Jackson, CR ;

Fedorka-Cray, PJ ;

Barrett, JB .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) :3558-3565

[8] Microfluidic Device for Efficient Airborne Bacteria Capture and Enrichment [J].

Jing, Wenwen ;

Zhao, Wang ;

Liu, Sixiu ;

Li, Lin ;

Tsai, Chi-Tay ;

Fan, Xiaoyong ;

Wu, Wenjuan ;

Li, Jingyan ;

Yang, Xin ;

Sui, Guodong .

ANALYTICAL CHEMISTRY, 2013, 85 (10) :5255-5262

[9] A field comparison of four samplers for enumerating fungal aerosols I. Sampling characteristics [J].

Lee, KS ;

Bartlett, KH ;

Brauer, M ;

Stephens, GM ;

Black, WA ;

Teschke, K .

INDOOR AIR, 2004, 14 (05) :360-366

[10] Circumventing air bubbles in microfluidic systems and quantitative continuous-flow PCR applications [J].

Nakayama, Tsuyoshi ;

Kurosawa, Yasunori ;

Furui, Satoshi ;

Kerman, Kagan ;

Kobayashi, Masaaki ;

Rao, S. Ramachandra ;

Yonezawa, Yuji ;

Nakano, Kouichi ;

Hino, Akihiro ;

Yamamura, Shohei ;

Takamura, Yuzuru ;

Tamiya, Eiichi .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 386 (05) :1327-1333

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