Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum β-lactamase genes

被引:10
作者
al Naiemi, Nashwan [1 ]
Schipper, Kim [1 ]
Duim, Birgitta [1 ]
Bart, Aldert [1 ]
机构
[1] Acad Med Ctr, Dept Med Microbiol, NL-1100 DD Amsterdam, Netherlands
关键词
D O I
10.1128/JCM.44.5.1896-1898.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Nucleotide sequencing is the standard molecular method for determination of the beta-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla(SHV) genes, as illustrated by three strains of three different species that each contained two different bla(SHV) alleles, SHV-2 and SHV-12.
引用
收藏
页码:1896 / 1898
页数:3
相关论文
共 14 条
[1]   Widespread transfer of resistance genes between bacterial species in an intensive care unit: Implications for hospital epidemiology [J].
Al Naiemi, N ;
Duim, B ;
Savelkoul, PHM ;
Spanjaard, L ;
de Jonge, E ;
Bart, A ;
Vandenbroucke-Grauls, CA ;
de Jong, MD .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (09) :4862-4864
[2]   A STANDARD NUMBERING SCHEME FOR THE CLASS-A BETA-LACTAMASES [J].
AMBLER, RP ;
COULSON, AFW ;
FRERE, JM ;
GHUYSEN, JM ;
JORIS, B ;
FORSMAN, M ;
LEVESQUE, RC ;
TIRABY, G ;
WALEY, SG .
BIOCHEMICAL JOURNAL, 1991, 276 :269-270
[3]   Automated thermal cycling is superior to traditional methods for nucleotide sequencing of blaSHV genes [J].
Bradford, PA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (12) :2960-2963
[4]   Extended-spectrum β-lactamases in the 21st century:: Characterization, epidemiology, and detection of this important resistance threat [J].
Bradford, PA .
CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (04) :933-951
[5]   Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].
Ewing, B ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :186-194
[6]  
HULETSKY A, 1993, J BIOL CHEM, V268, P3690
[7]   Mechanisms of disease:: The new β-lactamases [J].
Jacoby, GA ;
Munoz-Price, LS .
NEW ENGLAND JOURNAL OF MEDICINE, 2005, 352 (04) :380-391
[8]   Survey of Klebsiella pneumoniae strains producing extended-spectrum β-lactamases:: Prevalence of SHV-12 and SHV-2a in Korea [J].
Kim, J ;
Kwon, Y ;
Pai, H ;
Kim, JW ;
Cho, DT .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (05) :1446-1449
[9]   BETA-LACTAMASES IN LABORATORY AND CLINICAL RESISTANCE [J].
LIVERMORE, DM .
CLINICAL MICROBIOLOGY REVIEWS, 1995, 8 (04) :557-&
[10]   Extended-spectrum β-lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries:: Dominance and widespread prevalence of SHV- and CTX-M-type β-lactamases [J].
Paterson, DL ;
Hujer, KM ;
Hujer, AM ;
Yeiser, B ;
Bonomo, MD ;
Rice, LB ;
Bonomo, RA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (11) :3554-3560