Promoter Methylation of GATA4, WIF1, NTRK1 and Other Selected Tumour Suppressor Genes in Ovarian Cancer

被引：0
|
作者：
Chmelarova, M. [1 ]
Dvorakova, E. [2 ]
Spacek, J. [2 ]
Laco, J. [3 ,4 ]
Mzik, M. [1 ]
Palicka, V. [1 ]
机构：
[1] Charles Univ Prague, Fac Med Hradec Kralove, Inst Clin Biochem & Diagnost, CR-11636 Prague 1, Czech Republic
[2] Charles Univ Prague, Fac Med Hradec Kralove, Dept Obstet & Gyneacol, CR-11636 Prague 1, Czech Republic
[3] Charles Univ Prague, Fac Med Hradec Kralove, Fingerland Dept Pathol, CR-11636 Prague 1, Czech Republic
[4] Univ Hosp Hradec Kralove, Hradec Kralove 50005, Czech Republic
来源：
FOLIA BIOLOGICA | 2013年 / 59卷 / 02期
关键词：
MS-MLPA; DNA methylation; ovarian cancer; epigenetics; INHIBITORY FACTOR-I; LUNG-CANCER; HYPERMETHYLATION; CARCINOMAS; HEREDITARY; BRCA2;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Ovarian cancer is the leading cause of death from gynaecologic tumours, but the molecular and especially epigenetic events underlying the transformation are poorly understood. Various methylation changes have been identified and show promise as potential cancer biomarkers. The aim of this study was to investigate promoter methylation of selected tumour suppressor genes in ovarian cancer by comparison with normal ovarian tissue. To search for epigenetic events we used methylation-specific multiplex ligation-dependent probe amplification to compare the methylation status of 44 tissue samples of ovarian cancer with 30 control samples. Using a 20% cut-off for methylation, we observed significantly higher methylation in genes NTKR1, GATA4 and WIF1 in the ovarian cancer group compared with the control group. These findings could potentially be used in screening of ovarian cancer, and may have implications for future chemotherapy based on epigenetic changes.
引用
收藏
页码：87 / 92
页数：6
相关论文
共 48 条
  • [21] Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation
    Ramesh Ummanni
    Edgar Jost
    Melanie Braig
    Frithjof Lohmann
    Frederike Mundt
    Christine Barett
    Thorsten Schlomm
    Guido Sauter
    Tina Senff
    Carsten Bokemeyer
    Holger Sültmann
    Catherine Meyer-Schwesinger
    Tim H Brümmendorf
    Stefan Balabanov
    Molecular Cancer, 10
  • [22] Ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) is a potential tumour suppressor in prostate cancer and is frequently silenced by promoter methylation
    Ummanni, Ramesh
    Jost, Edgar
    Braig, Melanie
    Lohmann, Frithjof
    Mundt, Frederike
    Barett, Christine
    Schlomm, Thorsten
    Sauter, Guido
    Senff, Tina
    Bokemeyer, Carsten
    Sueltmann, Holger
    Meyer-Schwesinger, Catherine
    Bruemmendorf, Tim H.
    Balabanov, Stefan
    MOLECULAR CANCER, 2011, 10
  • [23] Methylation of tumour suppressor genes APAF-1 and DAPK-1 and in vitro effects of demethylating agents in bladder and kidney cancer
    Christoph, F.
    Kempkensteffen, C.
    Weikert, S.
    Koellermann, J.
    Krause, H.
    Miller, K.
    Schostak, M.
    Schrader, M.
    BRITISH JOURNAL OF CANCER, 2006, 95 (12) : 1701 - 1707
  • [24] Methylation of tumour suppressor genes APAF-1 and DAPK-1 and in vitro effects of demethylating agents in bladder and kidney cancer
    F Christoph
    C Kempkensteffen
    S Weikert
    J Köllermann
    H Krause
    K Miller
    M Schostak
    M Schrader
    British Journal of Cancer, 2006, 95 : 1701 - 1707
  • [25] Tumour-specific distribution of BRCA1 promoter region methylation supports a pathogenetic role in breast and ovarian cancer
    Bianco, T
    Chenevix-Trench, G
    Walsh, DCA
    Cooper, JE
    Dobrovic, A
    CARCINOGENESIS, 2000, 21 (02) : 147 - 151
  • [26] Promoter hypermethylation of MGMT, CDH1, RAR-β and SYK tumour suppressor genes in granulosa cell tumours (GCTs) of ovarian origin
    Dhillon, VS
    Young, AR
    Husain, SA
    Aslam, M
    BRITISH JOURNAL OF CANCER, 2004, 90 (04) : 874 - 881
  • [27] Promoter hypermethylation of MGMT, CDH1, RAR-β and SYK tumour suppressor genes in granulosa cell tumours (GCTs) of ovarian origin
    V S Dhillon
    A R Young
    S A Husain
    M Aslam
    British Journal of Cancer, 2004, 90 : 874 - 881
  • [28] GATA4, A NOVEL TUMOUR SUPPRESSOR GENE IN GLIOBLASTOMA MULTIFORME, MEDIATES ITS EFFECT THROUGH P1SINK4B AND P21CIP INDUCTION
    Agnihotri, Sameer
    Hawkins, Cynthia
    Guha, A.
    NEURO-ONCOLOGY, 2008, 10 (05) : 769 - 770
  • [29] Promoter methylation status of tumor suppressor genes and inhibition of expression of DNA methyltransferase 1 in non-small cell lung cancer
    Liu, Bangqing
    Song, Jianfei
    Luan, Jiaqiang
    Sun, Xiaolin
    Bai, Jian
    Wang, Haiyong
    Li, Angui
    Zhang, Lifei
    Feng, Xiaoyan
    Du, Zhenzong
    EXPERIMENTAL BIOLOGY AND MEDICINE, 2016, 241 (14) : 1531 - 1539
  • [30] Association Between Cyclin D1 Polymorphism with CpG Island Promoter Methylation Status of Tumor Suppressor Genes in Gastric Cancer
    Tahara, Tomomitsu
    Shibata, Tomoyuki
    Nakamura, Masakatsu
    Yamashita, Hiromi
    Yoshioka, Daisuke
    Okubo, Masaaki
    Yonemura, Joh
    Maeda, Yoshiteru
    Maruyama, Naoko
    Kamano, Toshiaki
    Kamiya, Yoshio
    Fujita, Hiroshi
    Nakagawa, Yoshihito
    Nagasaka, Mitsuo
    Iwata, Masami
    Hirata, Ichiro
    Arisawa, Tomiyasu
    DIGESTIVE DISEASES AND SCIENCES, 2010, 55 (12) : 3449 - 3457
12345