Production of bioactive peptide hydrolysates from deer, sheep, pig and cattle red blood cell fractions using plant and fungal protease preparations

被引：61
作者：
Bah, Clara S. F. [1 ]
Carne, Alan [2 ]
McConnell, Michelle A. [3 ]
Mros, Sonya [3 ]
Bekhit, Alaa El-Din A. [1 ]
机构：
[1] Univ Otago, Dept Food Sci, POB 56, Dunedin, New Zealand
[2] Univ Otago, Dept Biochem, Dunedin, New Zealand
[3] Univ Otago, Dept Microbiol & Immunol, Dunedin, New Zealand
来源：
FOOD CHEMISTRY | 2016年 / 202卷
关键词：
Animal blood; Red blood cell fraction; Plant protease; Fungal protease; Hydrolysis; Antioxidant activity; TOTAL ANTIOXIDANT CAPACITY; BOVINE HEMOGLOBIN; OPIOID-PEPTIDES; ANTIMICROBIAL PEPTIDES; ANTIBACTERIAL PEPTIDE; ASSAY;
D O I：
10.1016/j.foodchem.2016.02.020
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Protease preparations from plant (papain and bromelain) and fungal (FP400 and FPII) sources were used to hydrolyze the red blood cell fractions (RBCFs) separated from deer, sheep, pig, and cattle abattoir-sourced blood. After 1, 2, 4 and 24 h of hydrolysis, the antioxidant and antibacterial activities of the peptide hydrolysates obtained were investigated. The increase in trichloroacetic acid-soluble peptides over the hydrolysis period was examined using the o-phthaldialdehyde (OPA) assay and the hydrolysis profiles were illustrated using SDS-PAGE. Papain generated RBCF hydrolysates exhibited higher ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) compared to those generated with bromelain, FP400 and FPII. At certain concentrations, 24 h hydrolysates of RBCF using FP400 and FPII were able to inhibit the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results indicated that the use of proteases from plant or fungal sources can produce animal blood hydrolysates with antioxidant and antimicrobial activities. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：458 / 466
页数：9
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