Functional delivery of a cytosolic tRNA into mutant mitochondria of human cells

被引：81

作者：

Mahata, Bidesh ^{[1
]}

Mukherjee, Saikat ^{[1
]}

Mishra, Sumita ^{[1
]}

Bandyopadhyay, Arun ^{[1
]}

Adhya, Samit ^{[1
]}

机构：

[1] Indian Inst Chem Biol, Kolkata 700032, W Bengal, India

来源：

SCIENCE | 2006年 / 314卷 / 5798期

关键词：

D O I：

10.1126/science.1129754

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many maternally inherited and incurable neuromyopathies are caused by mutations in mitochondrial (mt) transfer RNA ( tRNA) genes. Kinetoplastid protozoa, including Leishmania, have evolved specialized systems for importing nucleus-encoded tRNAs into mitochondria. We found that the Leishmania RNA import complex (RIC) could enter human cells by a caveolin-1-dependent pathway, where it induced import of endogenous cytosolic tRNAs, including tRNALys, and restored mitochondrial function in a cybrid harboring a mutant mt tRNALys (MT-TK) gene. The use of protein complexes to modulate mitochondrial function may help in the management of such genetic disorders.

引用

页码：471 / 474

页数：4

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