Targeted Discovery and Combinatorial Biosynthesis of Polycyclic Tetramate Macrolactam Combamides A-E

被引：36

作者：

Liu, Yan ^{[1
]}

Wang, Haoxin ^{[2
]}

Song, Rentai ^{[2
]}

Chen, Jining ^{[1
]}

Li, Tianhong ^{[1
]}

Li, Yaoyao ^{[1
]}

Du, Liangcheng ^{[2
,3
]}

Shen, Yuemao ^{[1
,2
]}

机构：

[1] Shandong Univ, Sch Pharmaceut Sci, Key Lab Chem Biol, Minist Educ, Jinan 250012, Shandong, Peoples R China

[2] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China

[3] Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA

来源：

ORGANIC LETTERS | 2018年 / 20卷 / 12期

关键词：

MARINE SPONGE; LYSOBACTER-ENZYMOGENES; IKARUGAMYCIN; ACTIVATION; CYCLIZATION;

D O I：

10.1021/acs.orglett.8b01285

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Polycyclic tetramate macrolactams (PoTeMs) are a growing class of natural products with distinct structure and diverse biological activities. By promoter engineering and heterologous expression of the cryptic cbm gene cluster, four new PoTeMs, combamides A-E (1-4), were identified. Additionally, two new derivatives, combamides E (5) and F (6), were generated via combinatorial biosynthesis. Together, our findings provide a sound base for expanding the structure diversities of PoTeMs through genome mining and combinatorial biosynthesis.

引用

页码：3504 / 3508

页数：5

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