Exploring new marine bacterial species, Alcaligenes faecalis Alca F2018 valued for bioconversion of shrimp chitin to chitosan for concomitant biotechnological applications

被引:32
作者
Amer, Ohood A. [1 ]
Ali, Sameh S. [1 ,2 ]
Azab, Maha [1 ]
El-Shouny, Wagih A. [1 ]
Sun, Jianzhong [2 ]
Mahmoud, Yehia A. -G [1 ]
机构
[1] Tanta Univ, Fac Sci, Bot Dept, Tanta 31527, Egypt
[2] Jiangsu Univ, Biofuels Inst, Sch Environm & Safety Engn, Xuefu Rd 301, Zhenjiang 212013, Jiangsu, Peoples R China
关键词
Chitosan; Chitin deacetylase; Chitin; METARHIZIUM-ANISOPLIAE; ENTOMOPATHOGENIC FUNGI; DEACETYLASE; PURIFICATION; OPTIMIZATION; IMMOBILIZATION; PROTEIN;
D O I
10.1016/j.ijbiomac.2021.12.033
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The exploitation of chitinous materials seems to be an infinite treasure. To this end, using shellfish waste as the sole carbon/nitrogen source solves environmental challenges while lowering microbial chitinase production costs. Bioconversion of shellfish chitin wastes such as shrimp shells has recently been investigated for the production of enzymes and bioactive materials in order to maximize the utilization of chitin-containing seafood processing wastes. In this study, the bioconversion of chitin to chitosan by Alcaligenes faecalis Alca F2018 revealed the highest chitin deacetylase (CDA) activity of 40.6 U/mu g. The resulted low Km and high Vmax values explain the high affinity of the purified CDA to the p-nitroacetanilide substrate. CDA with a molecular weight of 66 KDa was purified from F2018 strain, with a 14.5% yield. FT-IR revealed distinct chitosan peaks and XRD revealed that chitosan samples had lower crystallinity than chitin. TGA analysis revealed that the recovered chitosan samples were more thermally stable. The deacetylation degree percentages of the produced chitosan are in the same range as that of the commercial chitosan, suggesting the promising potential of A. faecalis Alca F2018 to utilize shrimp shells in their raw form in the fermentation media based on its CDA enzyme activity.
引用
收藏
页码:35 / 45
页数:11
相关论文
共 66 条
[1]   Pharmaceutical Potential of a Novel Chitosan Derivative Schiff Base with Special Reference to Antibacterial, Anti-Biofilm, Antioxidant, Anti-Inflammatory, Hemocompatibility and Cytotoxic Activities [J].
Ali, Sameh S. ;
Kenawy, El-Refaie ;
Sonbol, Fatma I. ;
Sun, Jianzhong ;
Al-Etewy, Marwa ;
Ali, Asmaa ;
Huizi, Liu ;
El-Zawawy, Nessma A. .
PHARMACEUTICAL RESEARCH, 2019, 36 (01)
[2]   Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity [J].
Annamalai, Neelamegam ;
Rajeswari, Mayavan Veeramuthu ;
Vijayalakshmi, Shanmugam ;
Balasubramanian, Thangavel .
ANNALS OF MICROBIOLOGY, 2011, 61 (04) :801-807
[3]   PATHWAY OF CHITOSAN FORMATION IN MUCOR-ROUXII - ENZYMATIC DEACETYLATION OF CHITIN [J].
ARAKI, Y ;
ITO, E .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 55 (01) :71-78
[4]   Ethanol production from chitosan by the nematophagous fungus Pochonia chlamydosporia and the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana [J].
Aranda-Martinez, Almudena ;
Naranjo Ortiz, Miguel Angel ;
Abihssira Garcia, Isabel Sofia ;
Zavala-Gonzalez, Ernesto A. ;
Vicente Lopez-Llorca, Luis .
MICROBIOLOGICAL RESEARCH, 2017, 204 :30-39
[5]   Chitin and Chitosan as Direct Compression Excipients in Pharmaceutical Applications [J].
Badwan, Adnan A. ;
Rashid, Iyad ;
Al Omari, Mahmoud M. H. ;
Darras, Fouad H. .
MARINE DRUGS, 2015, 13 (03) :1519-1547
[6]   Heterologous expression and characterization of a novel chitin deacetylase, CDA3, from the mushroom Coprinopsis cinerea [J].
Bai, Yang ;
Wang, Yanxin ;
Liu, Xiao ;
Zhao, Jing ;
Kang, LiQin ;
Liu, Zhonghua ;
Yuan, Sheng .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 150 :536-545
[7]   Effect of deproteination and deacetylation conditions on viscosity of chitin and chitosan extracted from Crangon crangon shrimp waste [J].
Bajaj, Mini ;
Winter, Josef ;
Gallert, Claudia .
BIOCHEMICAL ENGINEERING JOURNAL, 2011, 56 (1-2) :51-62
[8]   Marine waste to a functional biomaterial: Green facile synthesis of modified-β-chitin from Uroteuthis duvauceli pens (gladius) [J].
Balitaan, Jolleen Natalie, I ;
Yeh, Jui-Ming ;
Santiago, Karen S. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 154 :1565-1575
[9]   Chitosan-based drug delivery systems [J].
Bernkop-Schnuerch, Andreas ;
Duennhaupt, Sarah .
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2012, 81 (03) :463-469
[10]   Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste [J].
Cahu, Thiago B. ;
Santos, Suzan D. ;
Mendes, Aline ;
Cordula, Carolina R. ;
Chavante, Suely F. ;
Carvalho, Luiz B., Jr. ;
Nader, Helena B. ;
Bezerra, Ranilson S. .
PROCESS BIOCHEMISTRY, 2012, 47 (04) :570-577