Hollow-Fiber Membrane Chamber as a Device for In Situ Environmental Cultivation

被引：86

作者：

Aoi, Yoshiteru ^{[1
]}

Kinoshita, Tomoyuki ^{[2
]}

Hata, Toru ^{[2
]}

Ohta, Hiroaki ^{[3
]}

Obokata, Haruko ^{[3
]}

Tsuneda, Satoshi ^{[3
]}

机构：

[1] Waseda Univ, Waseda Inst Adv Study, Shinjuku Ku, Tokyo 1628480, Japan

[2] Waseda Univ, Dept Chem Engn, Shinjuku Ku, Tokyo 1698555, Japan

[3] Waseda Univ, Dept Life Sci & Med Biosci, Shinjuku Ku, Tokyo 1628480, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 11期

关键词：

UNCULTURED SOIL BACTERIA; MICROBIAL DIVERSITY; ACTIVATED-SLUDGE; RIBOSOMAL-RNA; PURE CULTURE; MICROORGANISMS; GROWTH; CULTURABILITY; INCUBATION; CELLS;

D O I：

10.1128/AEM.02542-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

引用

页码：3826 / 3833

页数：8

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