Polyaniline Based Dual System for Potentiometric or UV-VIS Spectrophotometric Alkaline Phosphatase Detection

A simple disposable conducting polymer - polyaniline based system for the alkaline phosphatase activity determination either in potentiometric or UV-VIS spectrophotometric setup is described. The conducting polymer was placed on a plastic polyacetate Support by dispersing polyaniline nanoparticles. The detection method used is based on the enzymatic hydrolysis of monofluorophosphate which leads to a decrease of sample pH and finally results in a measurable change in the polyaniline electrode potential or the absorption spectra of the polymer. Both techniques offer similar sensitivity, but for the potentiometric mode a lower detection limit was obtained; it was close to 30 U L-1 (below 1 mg L-1).