A novel thermostable, alkaline pectate lyase from Bacillus tequilensis SV11 with potential in textile industry

被引：38

作者：

Chiliveri, Swarupa Rani ^{[1
]}

Linga, Venkateswar Rao ^{[1
]}

机构：

[1] Osmania Univ, Dept Microbiol, Hyderabad 500007, Andhra Pradesh, India

来源：

CARBOHYDRATE POLYMERS | 2014年 / 111卷

关键词：

Pectate lyase; Pectin; Bacillus; Bioscouring; MALDI-TOF; PURIFICATION; PECTINASE; EXPRESSION; BACTERIUM; PROTEINS;

D O I：

10.1016/j.carbpol.2014.04.065

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An extracellular pectate lyase was purified and characterized from a UV mutant of Bacillus tequilensis SV11. Purification resulted in a 16.2-fold improvement in the enzyme specific activity, with approximately 40.2% yield. SDS-PAGE showed that the enzyme had two subunits with molecular masses of 135 +/- 2 and 43 +/- 2 kDa. Further, MALDI-TOF MS experiments revealed that the mass spectrum of the second peptide significantly (91% score) matched with the unsaturated rhamnogalacturonyl hydrolase YteR OS-Bacillus subtilis (strain 168) by 27% sequence coverage, nominal mass 43,231 Da, and PI 5.91. The enzyme was optimally active at 60 degrees C, pH 9. K-m and V-max of the purified pectate lyase was found to be 1.220 mg/mL and 1773 U/mL, respectively. The enzyme was studied for its applicability in bioscouring and found to be efficient in the removal of 97.91% pectin of cotton fabric when compared with alkali-treated fabric. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：264 / 272

页数：9

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