Development of an oral mucosa equivalent using a porcine dermal matrix

被引：3

作者：

Basso, F. G. ^{[1
]}

Hebling, J. ^{[2
]}

Marcelo, C. L. ^{[3
]}

Costa, C. A. de Souza ^{[1
]}

Feinberg, S. E. ^{[3
]}

机构：

[1] Univ Estadual Paulista, Araraquara Dent Sch, Dept Physiol & Pathol, Sao Paulo, Brazil

[2] Univ Estadual Paulista, Araraquara Dent Sch, Dept Pediat Dent, Sao Paulo, Brazil

[3] Univ Michigan Hlth Syst, Dept Oral & Maxillofacial Surg, Ann Arbor, MI USA

来源：

BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY | 2017年 / 55卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

Oral keratinocytes; organotypic cell culture; oral mucosa; KERATINOCYTES;

D O I：

10.1016/j.bjoms.2016.09.019

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an exvivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell((R)) inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices. (C) 2016 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

引用

页码：308 / 311

页数：4

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