Optical detection of thymine dinucleoside monophosphate and its cis-syn photodimer by inorganic nanoparticles

被引：12
作者：
Caswell, KK
Mahtab, R
Murphy, CJ
机构：
[1] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA
[2] S Carolina State Univ, Dept Phys Sci, Orangeburg, SC 29117 USA
来源：
JOURNAL OF FLUORESCENCE | 2004年 / 14卷 / 04期
关键词：
thymine; photodimer; CdS; luminescence; SERS;
D O I：
10.1023/B:JOFL.0000031822.12344.84
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The Watson-Crick DNA double helix is an averaged ideal of multitudinous natural sequence-directed local structural deviations. By effectively derailing normal cellular physiological processes, damaged bases can induce noncanonical irregularities in the local structure of DNA if not efficiently repaired. Pyrimidine bases, especially thymine, are prone to dimerization when exposed to ultraviolet light. A [2 + 2] photocyclo-addition between adjacent thymine bases predominantly produces the cis-syn photodimer. These lesions, implicated in skin cancer, bend DNA by similar to30degrees due to their structural and conformational changes. Such changes in molecular properties can be detected by differential quenching of CdS nanoparticle luminescence and by surface-enhanced Raman scattering spectroscopy on metal nanoparticle substrates.
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收藏
页码：407 / 415
页数：9
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