ADI1, a methionine salvage pathway enzyme, is required for Drosophila fecundity

被引:17
作者
Chou, He-Yen [1 ]
Lin, Yu-Hung [1 ]
Shiu, Guan-Lin [1 ]
Tang, Hsiang-Yu [4 ,5 ]
Cheng, Mei-Ling [4 ,5 ]
Shiao, Ming-Shi [4 ,5 ]
Pai, Li-Mei [1 ,2 ,3 ]
机构
[1] Chang Gung Univ, Grad Inst Biomed Sci, Taoyuan, Taiwan
[2] Chang Gung Univ, Dept Biochem, Taoyuan, Taiwan
[3] Chang Gung Univ, Chang Gung Mol Med Res Ctr, Taoyuan, Taiwan
[4] Chang Gung Univ, Dept Biomed Sci, Taoyuan, Taiwan
[5] Chang Gung Univ, Coll Med, Hlth Aging Res Ctr, Taoyuan, Taiwan
关键词
ADI1; Methionine; MTA cycle; Fecundity; Drosophila; TAIL-BINDING PROTEIN-1; AMINO-ACID-METABOLISM; S-ADENOSYLMETHIONINE; POLYAMINE BIOSYNTHESIS; DIET RESTRICTION; GENE-EXPRESSION; LIFE-SPAN; SULFUR; METHYLTHIOADENOSINE; MELANOGASTER;
D O I
10.1186/s12929-014-0064-4
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background: Methionine, an essential amino acid, is required for protein synthesis and normal cell metabolism. The transmethylation pathway and methionine salvage pathway (MTA cycle) are two major pathways regulating methionine metabolism. Recently, methionine has been reported to play a key role in Drosophila fecundity. Results: Here, we revealed that the MTA cycle plays a crucial role in Drosophila fecundity using the mutant of aci-reductone dioxygenase 1 (DADI1), an enzyme in the MTA cycle. In dietary restriction condition, the egg production of adi1 mutant flies was reduced compared to that of control flies. This fecundity defect in mutant flies was rescued by reintroduction of Dadi1 gene. Moreover, a functional homolog of human ADI1 also recovered the reproduction defect, in which the enzymatic activity of human ADI1 is required for normal fecundity. Importantly, methionine supply rescued the fecundity defect in Dadi1 mutant flies. The detailed analysis of Dadi1 mutant ovaries revealed a dramatic change in the levels of methionine metabolism. In addition, we found that three compounds namely, methionine, SAM and Methionine sulfoxide, respectively, may be required for normal fecundity. Conclusions: In summary, these results suggest that ADI1, an MTA cycle enzyme, affects fly fecundity through the regulation of methionine metabolism.
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页数:9
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共 40 条
[1]   Metabolic Characteristics and Importance of the Universal Methionine Salvage Pathway Recycling Methionine from 5′-Methylthioadenosine [J].
Albers, Eva .
IUBMB LIFE, 2009, 61 (12) :1132-1142
[2]   Methylthioadenosine [J].
Avila, MA ;
García-Trevijano, ER ;
Lu, SC ;
Corrales, FJ ;
Mato, JM .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2004, 36 (11) :2125-2130
[3]   Amino acids and the regulation of methyl balance in humans [J].
Brosnan, John T. ;
da Silva, Robin ;
Brosnan, Margaret E. .
CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, 2007, 10 (01) :52-57
[4]  
Brosnan JT, 2006, J NUTR, V136, p1636S, DOI 10.1093/jn/136.6.1636S
[5]   Modification of protein surface hydrophobicity and methionine oxidation by oxidative systems [J].
Chao, CC ;
Ma, YS ;
Stadtman, ER .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (07) :2969-2974
[6]   Methylthioadenosine and polyamine biosynthesis in a Saccharomyces cerevisiae meu1Δ mutant [J].
Chattopadhyay, MK ;
Tabor, CW ;
Tabor, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 343 (01) :203-207
[7]   293 Cells Over-Expressing Human ADI1 and CD81 Are Permissive for Serum-Derived Hepatitis C Virus Infection [J].
Cheng, Ju-Chien ;
Yeh, Yung-Ju ;
Pai, Li-Mei ;
Chang, Ming-Ling ;
Yeh, Chau-Ting .
JOURNAL OF MEDICAL VIROLOGY, 2009, 81 (09) :1560-1568
[8]   Initiation of protein synthesis in mammalian cells with codons other than AUG and amino acids other than methionine [J].
Drabkin, HJ ;
Rajbhandary, UL .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (09) :5140-5147
[9]   Juvenile hormone as a regulator of the trade-off between reproduction and life span in Drosophila melanogaster [J].
Flatt, Thomas ;
Kawecki, Tadeusz J. .
EVOLUTION, 2007, 61 (08) :1980-1991
[10]   Survival costs of reproduction in Drosophila [J].
Flatt, Thomas .
EXPERIMENTAL GERONTOLOGY, 2011, 46 (05) :369-375