Mitogen-activated protein kinase phosphatase 1 negatively regulates MAPK signaling in mouse hypothalamus

被引：7

作者：

Adachi, Koichi ^{[1
]}

Goto, Motomitsu ^{[1
]}

Onoue, Takeshi ^{[1
]}

Tsunekawa, Taku ^{[1
]}

Shibata, Miyuki ^{[1
]}

Hagimoto, Shigeru ^{[1
]}

Ito, Yoshihiro ^{[1
]}

Banno, Ryoichi ^{[1
]}

Suga, Hidetaka ^{[1
]}

Sugimura, Yoshihisa ^{[1
]}

Oiso, Yutaka ^{[1
]}

Arima, Hiroshi ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Endocrinol & Diabet, Showa Ku, Nagoya, Aichi 4668550, Japan

来源：

NEUROSCIENCE LETTERS | 2014年 / 569卷

关键词：

MKP-1; MAPK; Hypothalamus; Inflammation; DUAL-SPECIFICITY PHOSPHATASE; SUPRACHIASMATIC NUCLEUS; DIFFERENTIAL REGULATION; ORGANOTYPIC CULTURES; GENE-EXPRESSION; NEUROPEPTIDE-Y; TRANSCRIPTIONAL ACTIVATION; PARAVENTRICULAR NUCLEUS; INSULIN-RESISTANCE; ARCUATE NUCLEUS;

D O I：

10.1016/j.neulet.2014.03.032

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Mitogen-activated protein kinase phosphatase 1 (MKP-1) is shown to negatively regulate MAPK signaling in various peripheral tissues as well as the central nervous system such as cortex, striatum and hippocampus. In this study, we examined whether MKP-1 regulates MAPK signaling in the mouse hypothalamus. Intraperitoneal injection of TNF alpha significantly increased MKP-1 mRNA expression in paraventricular and arcuate nuclei in the hypothalamus. TNF alpha treatment induced increases in MKP-1 expression at both mRNA and protein levels, accompanied by the inactivation of MAPK signaling in mouse hypothalamic explants. Inhibition of MKP-1 by its inhibitor or siRNA increased MAPK activity in the explants. Our data indicate that MKP-1 negatively regulates MAPK signaling in the mouse hypothalamus. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：49 / 54

页数：6

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