Molecularly Imprinted Polymerized Methylene Green as a Platform for Electrochemical Sensing of Aptamer-Thrombin Interactions

被引：28

作者：

Evtugyn, Gennady

Porfireva, Anna

Ivanov, Alexey ^{[1
]}

Konovalova, Olga ^{[2
]}

Hianik, Tibor ^{[1
]}

机构：

[1] Comenius Univ, Dept Nucl Phys & Biophys, Bratislava 84248, Slovakia

[2] Kazan VI Lenin State Univ, Dept Opt & Nanophoton, Kazan 420008, Russia

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 11期

基金：

俄罗斯基础研究基金会;

关键词：

DNA; Aptamers; Thrombin; Molecularly imprinted polymers; Methylene green; Electrochemical impedance spectroscopy; STRANDED-DNA; APTASENSOR; BIOSENSORS; ELECTRODE; AFFINITY; SENSOR;

D O I：

10.1002/elan.200804556

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The biosensors based on DNA aptamers immobilized by electrostatic adsorption onto electropolymerized Methylene Green (MG)) imprinted with DNA have been developed and examined for thrombin detection in the range from 1 nM to 1 mu M using electrochemical impedance spectroscopy (EIS) and potentiometry. The participation of DNA in MG electropolymerization and imprints formation was proved by measurements of EIS and redox signals of neutral and charged indicators. Changes in the morphology of the layers were examined using atomic force microscopy (AFM). The addition of DNA at the electropolymerization stage followed by acidic treatment of the coating significantly improved the efficiency of electrostatic adsorption of the DNA aptamer and provided sensitive detection of thrombin with limit of detection 0.5 nM.

引用

页码：1272 / 1277

页数：6

共 37 条

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