Synthesis of structured phosphatidylcholine containing punicic acid by the lipase-catalyzed transesterification with pomegranate seed oil

被引：11

作者：

Chojnacka, Anna ^{[1
]}

Gladkowski, Witold ^{[1
]}

Gliszczynska, Anna ^{[1
]}

Niezgoda, Natalia ^{[1
]}

Kielbowicz, Grzegorz ^{[1
]}

Wawrzenczyk, Czeslaw ^{[1
]}

机构：

[1] Wroclaw Univ Environm & Life Sci, Dept Chem, Norwida 25, PL-50375 Wroclaw, Poland

来源：

CATALYSIS COMMUNICATIONS | 2016年 / 75卷

关键词：

Transesterification; Egg-yolk phosphatidylcholine; Pomegranate seed oil; Conjugated fatty acids; Punicic acid; Immobilized lipase; CONJUGATED LINOLENIC ACID; FATTY-ACIDS; PHOSPHOLIPIDS; OPTIMIZATION; LIPIDS; CLA;

D O I：

10.1016/j.catcom.2015.11.019

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Incorporation of conjugated linolenic acid (punicic acid; 18:3, 9c,11t,13c) into egg-yolk phosphatidylcholine (PC) in the lipase-catalyzed transesterification process was the aim of this work. Pomegranate seed oil (PSO) containing over 77% of punicic acid was used as an acyl donor and three commercially available immobilized lipases were examined as biocatalysts. The effects of enzyme load, reaction time and molar ratio of substrates (PC:PSO) on the incorporation of punicic acid into the modified PC were tested. In all experiments the best results were obtained using lipase B from Candida antarctica. In optimum conditions (20% of enzyme load; 1:3 PC:PSO molar ratio; 72 h), the incorporation of punicic acid into the sn-1 position of PC was 56%. Additionally, the total content of polyunsaturated fatty acids in modified PC was almost 50%. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：60 / 64

页数：5

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