A comparison of DNA extraction methods for food analysis

被引：102
作者：
Di Pinto, Angela [1 ]
Forte, VitoTony
Guastadisegni, Maria Corsignano
Martino, Carmela
Schena, Francesco Paolo
Tantillo, Giuseppina
机构：
[1] Univ Bari, Dipartimento Sanita & Benessere Anim, Fac Med Vet, I-70010 Bari, Italy
[2] Apuliabiotech SCRL, I-70010 Bari, Italy
来源：
FOOD CONTROL | 2007年 / 18卷 / 01期
关键词：
food; DNA extraction; DNA purification; PCR;
D O I：
10.1016/j.foodcont.2005.08.011
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In this paper, two DNA extraction and purification procedures for food analysis, Wizard (R) Magnetic DNA Purification for Food (Promega) and DNeasy (R) 'Tissue Kit (QIAGEN), have been compared concerning extraction efficiency, DNA purity and DNA suitability for amplification. The comparison of two extraction methods, in this study, has highlighted the efficiency of the Wizard (R) Magnetic DNA Purification approach for vegetable matrices while the revised DNeasy (R) Tissue Kit for complex and processed matrices. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：76 / 80
页数：5
相关论文
共 16 条
[1]   Comparison of seven RNA extraction methods on stool and shellfish samples prior to hepatitis A virus amplification [J].
Arnal, C ;
Ferré-Aubineau, V ;
Besse, B ;
Mignotte, B ;
Schwartzbrod, L ;
Billaudel, S .
JOURNAL OF VIROLOGICAL METHODS, 1999, 77 (01) :17-26
[2]   DETECTION OF ENTERIC VIRUSES IN OYSTERS BY USING THE POLYMERASE CHAIN-REACTION [J].
ATMAR, RL ;
METCALF, TG ;
NEILL, FH ;
ESTES, MK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (02) :631-635
[3]   Identification of cow's milk in "buffalo" cheese by duplex polymerase chain reaction [J].
Bottero, MT ;
Civera, T ;
Anastasio, A ;
Turi, RM ;
Rosati, S .
JOURNAL OF FOOD PROTECTION, 2002, 65 (02) :362-366
[4]  
Calvo JH, 2001, J ANIM SCI, V79, P2108
[5]   POLYETHYLENE-GLYCOL PRECIPITATION FOR RECOVERY OF PATHOGENIC VIRUSES, INCLUDING HEPATITIS-A VIRUS AND HUMAN ROTAVIRUS, FROM OYSTER, WATER, AND SEDIMENT SAMPLES [J].
LEWIS, GD ;
METCALF, TG .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (08) :1983-1988
[6]   Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs [J].
Lipp, M ;
Bluth, A ;
Eyquem, F ;
Kruse, L ;
Schimmel, H ;
Van den Eede, G ;
Anklam, E .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2001, 212 (04) :497-504
[7]   DNA-based methods for food authentication [J].
Lockley, AK ;
Bardsley, RG .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2000, 11 (02) :67-77
[8]   A quick and simple method for the identification of meat species and meat products by PCR assay [J].
Matsunaga, T ;
Chikuni, K ;
Tanabe, R ;
Muroya, S ;
Shibata, K ;
Yamada, J ;
Shinmura, Y .
MEAT SCIENCE, 1999, 51 (02) :143-148
[9]   Development and application of DNA analytical methods for the detection of GMOs in food [J].
Meyer, R .
FOOD CONTROL, 1999, 10 (06) :391-399
[10]  
MEYR R, 1996, LEBENSMITTEL WISSENS, V29, P1
12