Thyroid hormone and retinoic acid induce the synthesis of insulin-like growth factor-binding protein-4 in prepubertal pig Sertoli cells

被引:16
作者
Bardi, G [1 ]
Bottazzi, C [1 ]
Demori, I [1 ]
Palmero, S [1 ]
机构
[1] Univ Genoa, Dipartimento Biol Sperimentale Ambientale & Appli, Sezione Fisiol Gen & Comparata, I-16132 Genoa, Italy
关键词
D O I
10.1530/eje.0.1410637
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A large body of evidence suggests the existence of an intratesticular IGF system complete with ligands, receptors and binding proteins (IGFBPs); the aim of the present study was to evaluate tri-iodothyronine (T-3) and retinoic acid (RA) effects on IGFBP production by Sertoli cells. A significant dose-dependent increase in IGFBP-4 mRNA levels was observed in Sertoli cells cultured in the presence of physiological concentrations of T-3 or RA. This response was inhibited by cycloheximide, indicating that de novo protein synthesis is required, as well as by actinomycin D, suggesting that the increase in mRNA levels requires transcriptional activation. As shown by ligand blot assays the stimulatory effects of both agents on IGFBP-4 mRNA expression appears to be consistent with an enhanced synthesis and secretion of IGFBP-4, thus suggesting that the transcriptional response is transduced to the protein level. Our data establish an important direct role for T-3 and RA in regulating IGFBP-4 expression and consequently IGF activity at the testis level.
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页码:637 / 643
页数:7
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