Evaluation of thermal desorption and solvent elution combined with polymer entrainment for the analysis of volatiles released by leaves from midge (Dasineura tetensi) resistant and susceptible blackcurrant (Ribes nigrum L.) cultivars

被引:0
|
作者
Griffiths, DW [1 ]
Robertson, GW [1 ]
Birch, ANE [1 ]
Brennan, RM [1 ]
机构
[1] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland
关键词
leaf volatiles; thermal desorption; leaf curling midge; blackcurrant; Ribes nigrum;
D O I
10.1002/(SICI)1099-1565(199911/12)10:6<328::AID-PCA473>3.0.CO;2-S
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Polymer entrainment combined with solvent elution or thermal desorption, steam distillation and solvent surface extractions has been applied to leaves from midge-resistant and -susceptible blackcurrant cultivars, Irrespective of the methods used, the predominant compounds detected were terpene hydrocarbons, but only minor quantitative differences were found in the relative concentrations of the individual mono- and sesqui-terpenes identified. Both steam distillation and thermal desorption resulted in the formation of a number of artefacts, whilst the use of polymer entrainment revealed the presence of a number of compounds, including a homoterpene not detected by either steam distillation or leaf surface solvent extraction. The relative merits of the various methods used are discussed in relation to their potential value for the identification and quantification of leaf surface volatiles. Copyright (C) 1999 John Wiley & Sons, Ltd.
引用
收藏
页码:328 / 334
页数:7
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