High-speed stimulated hyperspectral Raman imaging using rapid acousto-optic delay lines

被引：59

作者：

Alshaykh, Mohammed S. ^{[1
]}

Liao, Chien-Sheng ^{[2
]}

Sandoval, Oscar E. ^{[1
]}

Gitzinger, Gregory ^{[3
]}

Forget, Nicolas ^{[3
]}

Leaird, Daniel E. ^{[1
]}

Cheng, Ji-Xin ^{[1
,2
,4
,5
,6
]}

Weiner, Andrew M. ^{[1
,6
]}

机构：

[1] Purdue Univ, Dept Elect & Comp Engn, 465 Northwestern Ave, W Lafayette, IN 47907 USA

[2] Purdue Univ, Weldon Sch Biomed Engn, 206 S Martin Jischke Dr, W Lafayette, IN 47907 USA

[3] Fastlite, 1900 Route Cretes, F-06560 Valbonne, France

[4] Purdue Univ, Ctr Canc Res, W Lafayette, IN 47907 USA

[5] Purdue Univ, Dept Chem, 560 Oval Dr, W Lafayette, IN 47907 USA

[6] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA

来源：

OPTICS LETTERS | 2017年 / 42卷 / 08期

关键词：

MULTIVARIATE CURVE RESOLUTION; SCATTERING MICROSCOPY; IN-VIVO; SPECTROSCOPY; PULSES; LASER; DISPERSION;

D O I：

10.1364/OL.42.001548

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Stimulated Raman scattering (SRS) is a powerful, label-free imaging technique that holds significant potential for medical imaging. To allow chemical specificity and minimize spectral distortion in the imaging of live species, a highspeed multiplex SRS imaging platform is needed. By combining a spectral focusing excitation technique with a rapid acousto-optic delay line, we demonstrate a hyperspectral SRS imaging platform capable of measuring a 3-dB spectral window of similar to 200 cm(-1) within 12.8 mu s with a scan rate of 30 KHz. We present hyperspectral images of a mixture of two different microsphere polymers as well as live fungal cells mixed with human blood. (C) 2017 Optical Society of America

引用

页码：1548 / 1551

页数：4

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