Interactions among Dendrolimus punctatus cypovirus proteins and identification of the genomic segment encoding its A-spike

被引：8
作者：
Cheng, Chuangang [1 ]
Shao, Yunpeng [1 ]
Su, Lan [1 ]
Zhou, Yin [1 ]
Sun, Xiulian [1 ]
机构：
[1] Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Peoples R China
来源：
JOURNAL OF GENERAL VIROLOGY | 2014年 / 95卷
关键词：
CYTOPLASMIC-POLYHEDROSIS-VIRUS; MOLECULAR CHARACTERIZATION; RNA INTERACTIONS; ATOMIC MODEL; MECHANISM; SEQUENCE; PROVIDES; REVEALS; REGIONS; BINDING;
D O I：
10.1099/vir.0.064022-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Revealing the interactions among cypovirus proteins would facilitate our understanding of the replication and assembly of this virus. In the present study, interactions among proteins encoded by the 10 segments of Dendrolimus punctatus cypovirus (DpCPV) were identified using yeast two-hybrid (Y2H) and far-Western blotting assays. In total, 24 pairs of interactions were detected. Twelve pairs of one-direction interactions, four pairs of binary interactions and four pairs of self-associations were identified in the Y2H assays. Another four pairs of interactions were identified by far-Western blotting. The interactions between the methyltransferase domain of the turret protein (VP3) and VP4 as well as between polyhedrin and VP4 were further confirmed by far-Western blotting and pull-down assays, respectively. In addition, immunogold labelling showed that the A-spike of DpCPV is formed by VP4. In conclusion, we obtained a protein protein interaction network of DpCPV and showed that its A-spike is formed by VP4 encoded by genomic segment 6.
引用
收藏
页码：1532 / 1538
页数：7
相关论文
共 27 条
[1]   Carboxyl-proximal regions of reovirus nonstructural protein μNS necessary and sufficient for forming factory-like inclusions [J].
Broering, TJ ;
Arnold, MM ;
Miller, CL ;
Hurt, JA ;
Joyce, PL ;
Nibert, ML .
JOURNAL OF VIROLOGY, 2005, 79 (10) :6194-6206
[2]   Functional investigation of grass carp reovirus nonstructural protein NS80 [J].
Cai, Lin ;
Sun, Xiaoyun ;
Shao, Ling ;
Fang, Qin .
VIROLOGY JOURNAL, 2011, 8
[3]   Molecular characterization of genome segments 1 and 3 encoding two capsid proteins of Antheraea mylitta cytoplasmic polyhedrosis virus [J].
Chakrabarti, Mrinmay ;
Ghorai, Suvankar ;
Mani, Saravana K. K. ;
Ghosh, Ananta K. .
VIROLOGY JOURNAL, 2010, 7
[4]   Genome segment 6 of Antheraea mylitta cypovirus encodes a structural protein with ATPase activity [J].
Chavali, Venkata R. M. ;
Madhurantakam, Chaithanya ;
Ghorai, Suvankar ;
Roy, Sobhan ;
Das, Amit K. ;
Ghosh, Ananta K. .
VIROLOGY, 2008, 377 (01) :7-18
[5]   Electron Tomography Reveals Polyhedrin Binding and Existence of both Empty and Full Cytoplasmic Polyhedrosis Virus Particles inside Infectious Polyhedra [J].
Chen, Justin ;
Sun, Jingchen ;
Atanasov, Ivo ;
Ryazantsev, Sergey ;
Zhou, Z. Hong .
JOURNAL OF VIROLOGY, 2011, 85 (12) :6077-6081
[6]   Characterization of the RNA-binding regions in protein p36 of Heliothis armigera cypovirus 14 [J].
Chen, Wuguo ;
Hu, Yuanyang ;
Li, Yang ;
Yu, Zheng ;
Dong, Changjin ;
Cai, Dawei ;
Zhang, Jiamin .
VIRUS RESEARCH, 2007, 125 (02) :211-218
[7]  
Chen WG, 2006, J BIOCHEM MOL BIOL, V39, P412
[8]   Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping [J].
Cheng, Lingpeng ;
Sun, Jingchen ;
Zhang, Kai ;
Mou, Zongjun ;
Huang, Xiaoxing ;
Ji, Gang ;
Sun, Fei ;
Zhang, Jingqiang ;
Zhu, Ping .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (04) :1373-1378
[9]   SIGMA-1 PROTEIN OF MAMMALIAN REOVIRUSES EXTENDS FROM THE SURFACES OF VIRAL PARTICLES [J].
FURLONG, DB ;
NIBERT, ML ;
FIELDS, BN .
JOURNAL OF VIROLOGY, 1988, 62 (01) :246-256
[10]   Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1 [J].
Hagiwara, K ;
Kobayashi, J ;
Tomita, M ;
Yoshimura, T .
ARCHIVES OF VIROLOGY, 2001, 146 (01) :181-187
123