Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system

被引:32
|
作者
Stahl, Bethany A. [1 ,2 ]
Peuss, Robert [3 ]
McDole, Brittnee [1 ,2 ]
Kenzior, Alexander [3 ]
Jaggard, James B. [1 ,2 ]
Gaudenz, Karin [3 ]
Krishnan, Jaya [3 ]
McGaugh, Suzanne E. [4 ]
Duboue, Erik R. [2 ,5 ]
Keene, Alex C. [1 ,2 ]
Rohner, Nicolas [3 ,6 ]
机构
[1] Florida Atlantic Univ, Dept Biol Sci, 5353 Parkside Dr, Boca Raton, FL 33458 USA
[2] Florida Atlantic Univ, Jupiter Life Sci Initiat, Boca Raton, FL 33431 USA
[3] Stowers Inst Med Res, 1000 E 50th St, Kansas City, MO 64110 USA
[4] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA
[5] Florida Atlantic Univ, Wilkes Honors Coll, Jupiter, FL USA
[6] KU Med Ctr, Dept Mol & Integrat Physiol, Kansas City, KS USA
关键词
DEVELOPMENTAL BIOLOGY; LINKAGE MAP; CAVEFISH; CONVERGENCE; ADAPTATION; EXPRESSION; GOLDFISH; NUCLEUS; TOOLS;
D O I
10.1002/dvdy.32
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Background Astyanax mexicanus is a well-established fish model system for evolutionary and developmental biology research. These fish exist as surface forms that inhabit rivers and 30 different populations of cavefish. Despite important progress in the deployment of new technologies, deep mechanistic insights into the genetic basis of evolution, development, and behavior have been limited by a lack of transgenic lines commonly used in genetic model systems. Results Here, we expand the toolkit of transgenesis by characterizing two novel stable transgenic lines that were generated using the highly efficient Tol2 system, commonly used to generate transgenic zebrafish. A stable transgenic line consisting of the zebrafish ubiquitin promoter expresses enhanced green fluorescent protein ubiquitously throughout development in a surface population of Astyanax. To define specific cell-types, a Cntnap2-mCherry construct labels lateral line mechanosensory neurons in zebrafish. Strikingly, both constructs appear to label the predicted cell types, suggesting many genetic tools and defined promoter regions in zebrafish are directly transferrable to cavefish. Conclusion The lines provide proof-of-principle for the application of Tol2 transgenic technology in A. mexicanus. Expansion on these initial transgenic lines will provide a platform to address broadly important problems in the quest to bridge the genotype-phenotype gap.
引用
收藏
页码:679 / 687
页数:9
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