Identification of novel antioxidant peptides from sea squirt (Halocynthia roretzi) and its neuroprotective effect in 6-OHDA-induced neurotoxicity

被引:1
|
作者
Ma, Rui [1 ,2 ]
Chen, Qiqi [3 ,4 ]
Dai, Yanping [5 ]
Huang, Yan [3 ,4 ]
Hou, Qing [1 ,2 ]
Huang, Yuting [1 ,2 ]
Zhong, Kai [1 ,2 ]
Huang, Yina [3 ,4 ]
Gao, Hong [1 ,2 ]
Bu, Qian [1 ,2 ,3 ,4 ,5 ]
机构
[1] Sichuan Univ, Coll Biomass & Engn, Dept Food Sci & Technol, Chengdu 610065, Peoples R China
[2] Sichuan Univ, Hlth Food Evaluat Res Ctr, Chengdu 610065, Peoples R China
[3] Sichuan Univ, West China Sch Publ Hlth, Chengdu 610041, Peoples R China
[4] Sichuan Univ, West China Fourth Hosp, Chengdu 610041, Peoples R China
[5] Sichuan Univ, Natl Chengdu Ctr Safety Evaluat Drugs, State Key Lab BiotherapyCollaborat Innovat Ctr Bi, West China Hosp,West China Med Sch, Chengdu 610041, Peoples R China
关键词
IN-VITRO METHODS; OXIDATIVE STRESS; PURIFICATION; EXPRESSION; MODEL; APOPTOSIS; SYSTEMS; CELLS; TRP;
D O I
10.1039/d2fo00729k
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ocean life contains a wealth of bioactive peptides that could be utilized in nutraceuticals and pharmaceuticals. This study aimed to obtain neuroprotective antioxidant peptides in sea squirt (Halocynthia roretzi) through protamex enzymolysis. Fraction F4 (ultrafiltration generated four fractions) had a lower molecular weight (<500 Dalton (Da)) with greater 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities (94.24 +/- 2.50% and 91.80 +/- 1.19%). After gel filtration, six peptides, including Phe-Gly-Phe (FGF), Leu-Gly-Phe (LGF), Leu-Phe-VAL (LFV), Val-Phe-Leu (VFL), Trp-Leu-Pro (WLP), and Ile-Ser-Trp (ISW), were identified and sequenced by liquid chromatography-mass spectrometry (LC-MS/MS). Peptides WLP and ISW showed higher oxygen radical absorbance capacity (ORAC) values (2.72 +/- 0.47 and 1.93 +/- 0.01 mu mol L-1 of Trolox equivalent (TE) per mu mol L-1 of peptide) than glutathione (GSH). Additionally, WLP effectively increased cell viability, dramatically attenuated 6-Hydroxydopamine (6-OHDA)-induced cell apoptosis and decreased reactive oxygen species (ROS) levels to nearly two-fold, and significantly boosted glutathione peroxidase (GSH-Px) activity in PC12 cells. Transcriptome sequencing revealed differential expression of genes associated with various oxidative stress pathways after WLP treatment, such as glutathione metabolism. These results suggest that the Halocynthia roretzi-derived tripeptide WLP could alleviate neurodegenerative diseases associated with oxidative stress.
引用
收藏
页码:6008 / 6021
页数:14
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