Screening of Key Antioxidant Compounds of Longan (Dimocarpus longan Lour.) Seed Extract by Combining Online Fishing/Knockout, Activity Evaluation, Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, and High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry Methods

被引：30

作者：

Chen, Jinyu ^{[1
]}

Ge, Zhen-zhen ^{[1
]}

Zhu, Wei ^{[1
]}

Xu, Ze ^{[1
]}

Li, Chun-mei ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China

[2] Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Food Sci, Wuhan 430070, Hubei, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2014年 / 62卷 / 40期

关键词：

longan seed; phenolic compounds; online knockout; FT-ICR-MS; HPLC-ESI-MS; HPLC-ESI-MS; GALLIC ACID; POLYPHENOLIC COMPOUNDS; STRUCTURE ELUCIDATION; PHENOLIC CONTENT; ELLAGIC ACID; DPPH; COMPONENTS; CAPACITY; FRUIT;

D O I：

10.1021/jf502995z

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

To figure out the key phenolic compounds accounting for the antioxidant effects of longan (Dimocarpus longan Lour.) seed extract online fishing/knockout method, activity evaluation assays, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), and high-peformance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis were used jointly for the first time. p-Coumaric acid-glycoside, (S)-flavogallonic acid, ellagic acid derivative, and methyl ellagic acid glucopyranoside were first identified in longan seeds. In addition, our study revealed that ellagic acid well as its derivative and p-ourmaric acid-glycoside had important contribution to the potent antioxidant activity of longan seed extract, while gallic acid corilagin, (S)-flavogallonic acid, methyl-ellagic acid glucopyranoside, and ethyl gallate showed very little contribution to the total antioxidant activity of longan seed extract. The combining use of the online fishing/knockout method activity evaluation assays, FT-ICR-MS, and HPLC-ESI-MS anlaysis is a useful and simple strategy for screening of key bioactive compounds from complex extracts.

引用

页码：9744 / 9750

页数：7

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