Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors

被引：0

作者：

Steinmark, I. Emilie ^{[1
]}

James, Arjuna L. ^{[1
]}

Dreiss, Cecile A. ^{[2
]}

Yahioglu, Gokhan ^{[3
]}

Suhling, Klaus ^{[1
]}

机构：

[1] Kings Coll London, Dept Phys, London WC2R 2LS, England

[2] Kings Coll London, Inst Pharmaceut Sci, London SE1 9NH, England

[3] Imperial Coll London, Dept Chem, London, England

来源：

REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS XI | 2019年 / 10893卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

Fluorescent molecular rotors; viscosity; mitochondria; fluorescence lifetime imaging; DIFFUSION;

D O I：

10.1117/12.2508676

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.

引用

页数：7

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