Imaging mitochondrial matrix viscosity in live cells via Fluorescence Lifetime Imaging (FLIM) of fluorescent molecular rotors

被引：0
|
作者：
Steinmark, I. Emilie [1 ]
James, Arjuna L. [1 ]
Dreiss, Cecile A. [2 ]
Yahioglu, Gokhan [3 ]
Suhling, Klaus [1 ]
机构：
[1] Kings Coll London, Dept Phys, London WC2R 2LS, England
[2] Kings Coll London, Inst Pharmaceut Sci, London SE1 9NH, England
[3] Imperial Coll London, Dept Chem, London, England
来源：
REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS XI | 2019年 / 10893卷
基金：
英国生物技术与生命科学研究理事会;
关键词：
Fluorescent molecular rotors; viscosity; mitochondria; fluorescence lifetime imaging; DIFFUSION;
D O I：
10.1117/12.2508676
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.
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页数：7
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