Human Fibroblasts for Large-Scale "Omics" Investigations of ATM Gene Function

被引：8

作者：

Jung, Mira ^{[1
]}

Timofeeva, Olga ^{[2
]}

Cheema, Amrita K. ^{[2
]}

Varghese, Rency ^{[2
]}

Ressom, Habtom ^{[2
]}

Dritschilo, Anatoly ^{[1
,2
,3
]}

机构：

[1] Georgetown Univ, Med Ctr, Dept Radiat Med, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA

[2] Georgetown Univ, Med Ctr, Dept Oncol, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA

[3] Georgetown Univ, Med Ctr, Dept Radiat Med, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA

来源：

HUMAN CELL TRANSFORMATION: ROLE OF STEM CELLS AND THE MICROENVIRONMENT | 2012年 / 720卷

关键词：

ATAXIA-TELANGIECTASIA; RADIATION SENSITIVITY; KINASES; CELLS;

D O I：

10.1007/978-1-4614-0254-1_15

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

ATM (gene mutated in ataxia-telangiectasia) is a critical central component of the pleiotropic responses of cells to ionizing radiation-induced stress. To gain insight into molecular mechanisms and to enhance our understanding of ATM functions, we have advanced a human model cell system, derived from genetically defined immortal fibroblasts, and we have applied high-throughput genomic, proteomic and metabolomic technologies for a systems level analysis. The cellular characterizations reported here provide the background for application of a systems analysis to integrate transcription, post-translational modifications and metabolic activity induced by exposure of cells to ionizing radiation. We present here a summary of the derivation and characterization of cells comprising this model cell system and review applications of this model to systems analysis of ATM functions.

引用

页码：181 / 190

页数：10

共 13 条

[1] COMPUTER-PROGRAMS FOR THE ANALYSIS OF CELLULAR-SURVIVAL DATA [J].