The Radio-Sensitizing Effect of Pharmacological Concentration of Ascorbic Acid on Human Pancreatic Cancer Cells

被引：7
作者：
Dayer, Dian [1 ]
Tabandeh, Mohammad R. [2 ,3 ]
Kazemi, Majid [4 ]
机构：
[1] Ahvaz Jundishapur Univ Med Sci, Cellular & Mol Res Ctr, Ahvaz 61357831351, Iran
[2] Shahid Chamran Univ Ahvaz, Fac Vet Med, Dept Basic Sci, Div Biochem & Mol Biol, Ahvaz, Iran
[3] Shahid Chamran Univ Ahvaz, Stem Cells & Transgen Technol Res Ctr, Ahvaz, Iran
[4] Ahvaz Jundishapur Univ Med Sci, Med Fac, Dept Med Lab Sci, Ahvaz, Iran
来源：
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY | 2020年 / 20卷 / 16期
关键词：
Ascorbic acid; radiotherapy; pancreatic cancer; reactive oxygen species; Sax expression; Bcl2; expression; DNA fragmentation; HYDROGEN-PEROXIDE; VITAMIN-C; APOPTOSIS;
D O I：
10.2174/1871520620666200612144124
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Previous studies reported the inevitable destructive effects of radiotherapy on normal adjacent cells. Ascorbic Acid (AA) has been proposed as an effective anti-cancer agent with no obvious effects on normal cells. Objective: The effects of Ascorbic acid in combination with radiotherapy on human pancreatic carcinoma cell line were studied. Methods: The human pancreatic cancer cells were cultured and divided into four groups: control group (A) without any treatment, group B that received 2Gy radiotherapy alone, group C that was treated with 4mM AA alone, and group D that was co-treated with AA and radiotherapy. Cell viability, DNA fragmentation, expression of apoptotic genes, and Reactive Oxygen Species (ROS) production were determined in treated cells. Results: There was a noticeable decrease in cell viability after treatment with AA (and/or) radiotherapy. All treated groups showed elevated ROS production, Bax/Bcl2 expression, DNA fragmentation, and cytotoxycity compared with the control group. Cells under combination therapy showed the most cytotoxicity. Conclusion: The results suggest that AA at a dose of 4mmol/l may be used as an effective radio-sensitizing agent in pancreatic cancer cell line.
引用
收藏
页码：1927 / 1932
页数：6
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