The Radio-Sensitizing Effect of Pharmacological Concentration of Ascorbic Acid on Human Pancreatic Cancer Cells

被引：7

作者：

Dayer, Dian ^{[1
]}

Tabandeh, Mohammad R. ^{[2
,3
]}

Kazemi, Majid ^{[4
]}

机构：

[1] Ahvaz Jundishapur Univ Med Sci, Cellular & Mol Res Ctr, Ahvaz 61357831351, Iran

[2] Shahid Chamran Univ Ahvaz, Fac Vet Med, Dept Basic Sci, Div Biochem & Mol Biol, Ahvaz, Iran

[3] Shahid Chamran Univ Ahvaz, Stem Cells & Transgen Technol Res Ctr, Ahvaz, Iran

[4] Ahvaz Jundishapur Univ Med Sci, Med Fac, Dept Med Lab Sci, Ahvaz, Iran

来源：

ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY | 2020年 / 20卷 / 16期

关键词：

Ascorbic acid; radiotherapy; pancreatic cancer; reactive oxygen species; Sax expression; Bcl2; expression; DNA fragmentation; HYDROGEN-PEROXIDE; VITAMIN-C; APOPTOSIS;

D O I：

10.2174/1871520620666200612144124

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Previous studies reported the inevitable destructive effects of radiotherapy on normal adjacent cells. Ascorbic Acid (AA) has been proposed as an effective anti-cancer agent with no obvious effects on normal cells. Objective: The effects of Ascorbic acid in combination with radiotherapy on human pancreatic carcinoma cell line were studied. Methods: The human pancreatic cancer cells were cultured and divided into four groups: control group (A) without any treatment, group B that received 2Gy radiotherapy alone, group C that was treated with 4mM AA alone, and group D that was co-treated with AA and radiotherapy. Cell viability, DNA fragmentation, expression of apoptotic genes, and Reactive Oxygen Species (ROS) production were determined in treated cells. Results: There was a noticeable decrease in cell viability after treatment with AA (and/or) radiotherapy. All treated groups showed elevated ROS production, Bax/Bcl2 expression, DNA fragmentation, and cytotoxycity compared with the control group. Cells under combination therapy showed the most cytotoxicity. Conclusion: The results suggest that AA at a dose of 4mmol/l may be used as an effective radio-sensitizing agent in pancreatic cancer cell line.

引用

页码：1927 / 1932

页数：6

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