Stratification of clear cell renal cell carcinoma by signaling pathway analysis

被引：7

作者：

Cremona, Mattia ^{[1
]}

Espina, Virginia ^{[2
]}

Caccia, Dario ^{[1
]}

Veneroni, Silvia ^{[1
]}

Colecchia, Maurizio ^{[3
]}

Pierobon, Mariaelena ^{[2
]}

Deng, Jianghong ^{[2
]}

Mueller, Claudius ^{[2
]}

Procopio, Giuseppe ^{[4
]}

Lanzi, Cinzia ^{[1
]}

Daidone, Maria Grazia ^{[1
]}

Cho, William C. S. ^{[5
]}

Petricoin, Emanuel F. ^{[2
]}

Liotta, Lance ^{[2
]}

Bongarzone, Italia ^{[1
]}

机构：

[1] Fdn IRCCS Ist Nazl Tumori, Dept Expt Oncol & Mol Med, Milan, Italy

[2] George Mason Univ, Ctr Appl Prote & Mol Med, Manassas, VA USA

[3] Fdn IRCCS Ist Nazl Tumori, Dept Pathol, Milan, Italy

[4] Fdn IRCCS Ist Nazl Tumori, Dept Med Oncol, Milan, Italy

[5] Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Hong Kong, Peoples R China

来源：

EXPERT REVIEW OF PROTEOMICS | 2014年 / 11卷 / 02期

关键词：

autophagy; HIF; hypoxia; mTOR; renal cell carcinoma; reverse-phase protein array; signaling pathway; GROWTH-FACTOR RECEPTOR; TARGETED THERAPY; CANCER; AUTOPHAGY; MTOR; EGFR; VHL; PHOSPHORYLATION; MICRODISSECTION; INACTIVATION;

D O I：

10.1586/14789450.2014.893193

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Investigation of cell signaling pathways in 16 clear cell renal cell carcinomas to identify groups based on commonly shared phosphorylation-driven signaling networks. Using laser capture microdissection and reverse-phase protein arrays, we profiled 75 key nodes spanning signaling pathways important in tumorigenesis. Analysis revealed significantly different (P < 0.05) signaling levels for 27 nodes between two groups of samples, designated A (4 samples; high EGFR, RET, and RASGFR1 levels, converging to activate AKT/mTOR) and B (12 samples; high ERK1/2 and STAT phosphorylation). Group B was further partitioned into groups C (7 samples; elevated expression of LC3B) and D (5 samples; activation of Src and STAT). Network analysis indicated that group A was characterized by signaling pathways related to cell cycle and proliferation, and group B by pathways related to cell death and survival. Homogeneous clear cell renal cell carcinomas could be stratified into at least two major functional groups.

引用

页码：237 / 249

页数：13

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