Molecular Cloning of Grass Carp Growth Hormone Receptor and Its Functional Interaction with Silver Carp Growth Hormone

被引：7

作者：

Hu, Wei ^{[1
]}

Chen, Xia ^{[1
]}

Yu, Gusong ^{[1
]}

Chen, Yan ^{[1
]}

Shen, Yan ^{[1
]}

Zhang, Ying ^{[1
]}

Gong, Yi ^{[1
]}

机构：

[1] Chinese Acad Sci, Grad Univ, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China

来源：

PROTEIN AND PEPTIDE LETTERS | 2009年 / 16卷 / 03期

关键词：

cell proliferation; CHO-K1; cell; grass carp; growth hormone level; growth hormone receptor; SIGNAL-TRANSDUCTION; EXPRESSION; SEABREAM; FORMS; FISH;

D O I：

10.2174/092986609787601679

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (K-d) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein.

引用

页码：320 / 323

页数：4

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