Molecular Cloning of Grass Carp Growth Hormone Receptor and Its Functional Interaction with Silver Carp Growth Hormone

被引：7
|
作者：
Hu, Wei [1 ]
Chen, Xia [1 ]
Yu, Gusong [1 ]
Chen, Yan [1 ]
Shen, Yan [1 ]
Zhang, Ying [1 ]
Gong, Yi [1 ]
机构：
[1] Chinese Acad Sci, Grad Univ, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China
来源：
PROTEIN AND PEPTIDE LETTERS | 2009年 / 16卷 / 03期
关键词：
cell proliferation; CHO-K1; cell; grass carp; growth hormone level; growth hormone receptor; SIGNAL-TRANSDUCTION; EXPRESSION; SEABREAM; FORMS; FISH;
D O I：
10.2174/092986609787601679
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (K-d) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein.
引用
收藏
页码：320 / 323
页数：4
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