Identification of Crocus sativus and its Adulterants from Chinese Markets by using DNA Barcoding Technique

被引:31
作者
Huang, Wei-juan [1 ]
Li, Fei-fei [1 ]
Liu, Yu-jing [1 ]
Long, Chun-lin [1 ,2 ]
机构
[1] Minzu Univ China, Coll Life & Environm Sci, Dept Biol, Beijing 100081, Peoples R China
[2] Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
基金
中国国家自然科学基金;
关键词
Adulterant identification; Classification; Crocus sativus; DNA barcoding; LAND PLANTS; SAFFRON; AUTHENTICATION; L; SCAR; ITS2;
D O I
10.15171/ijb.1034
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Saffron (Crocus sativus L.) is a common but very expensive herbal medicine. As an important traditional medicine, it has an outstanding effect in treating irregular and painful menstruation. Recently, the over-demand tendency of saffron results in an unusual phenomenon in the medicinal markets. Adulterants and saffron-like substitutes are intentionally mixed into medicinal markets and pharmacies or online stores, affecting drug safety and food quality. Objectives: Our study aimed to identify saffron from its adulterants via DNA barcoding. Materials and Methods: Samples (13 saffron + 4 others containing Carthamus tinctorius or Chrysanthemum x morifolium) obtained from 12 different provinces of China. Through DNA barcoding, samples were compared using three candidate markers, trnH-psbA, rbcL-a and ITS2. Results: trnH-psbA and rbcL-a were capable of distinguishing different accessions. ITS2 could identify samples even at intra-specific level. According to these three barcodes, four samples were identified saffron-like substitutes. Conclusions: The adulterant rate in Chinese markets reaches as high as 33.33% that may cause health risks and further may reduce saffron efficacy once is being used as herbal remedy. In order to make a distinction between C. sativus with other genera as adulterants, DNA barcoding is suggested.
引用
收藏
页码:36 / 42
页数:7
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