Analysis of short-chain fatty acids in human feces: A scoping review

被引：124

作者：

Primec, Masa ^{[1
]}

Micetic-Turk, Dusanka ^{[2
]}

Langerholc, Tomaz ^{[1
]}

机构：

[1] Univ Maribor, Fac Agr & Life Sci, Dept Microbiol Biochem Mol Biol & Biotechnol, Pivola 10, Hoce 2311, Slovenia

[2] Univ Maribor, Fac Med, Dept Pediat, Taborska Ulica 8, SLO-2000 Maribor, Slovenia

来源：

ANALYTICAL BIOCHEMISTRY | 2017年 / 526卷

关键词：

Short-chain fatty acids (SCFAs); Feces; Human; Detection methods; Scoping review; PERFORMANCE LIQUID-CHROMATOGRAPHY; IRRITABLE-BOWEL-SYNDROME; RECEIVING ENTERAL NUTRITION; RANDOMIZED CONTROLLED-TRIAL; ACTIVE ULCERATIVE-COLITIS; TUBULAR CAPILLARY COLUMNS; PLACEBO-CONTROLLED TRIAL; VOLATILE ORGANIC-ACIDS; HEALTHY-HUMAN SUBJECTS; FECAL MICROBIOTA;

D O I：

10.1016/j.ab.2017.03.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Short-chain fatty acids (SCFAs) play a crucial role in maintaining homeostasis in humans, therefore the importance of a good and reliable SCFAs analytical detection has raised a lot in the past few years. The aim of this scoping review is to show the trends in the development of different methods of SCFAs analysis in feces, based on the literature published in the last eleven years in all major indexing databases. The search criteria included analytical quantification techniques of SCFAs in different human clinical and in vivo studies. SCFAs analysis is still predominantly performed using gas chromatography (GC), followed by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and capillary electrophoresis (CE). Performances, drawbacks and advantages of these methods are discussed, especially in the light of choosing a proper pretreatment, as feces is a complex biological material. Further optimization to develop a simple, cost effective and robust method for routine use is needed. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：9 / 21

页数：13

共 184 条

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