Landscape of genomic imprinting and its functions in the mouse mammary gland

被引：3

作者：

Xu, Haibo ^{[1
,2
]}

Zhao, Lina ^{[1
,2
]}

Feng, Xu ^{[1
]}

Ma, Yujie ^{[1
]}

Chen, Wei ^{[1
]}

Zou, Li ^{[1
]}

Yang, Qin ^{[1
]}

Sun, Jihong ^{[3
]}

Yu, Hong ^{[4
]}

Jiao, Baowei ^{[1
,5
,6
]}

机构：

[1] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China

[2] Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650223, Yunnan, Peoples R China

[3] Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Radiol, Sch Med, Hangzhou 310016, Peoples R China

[4] Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Gen Surg, Sch Med, Hangzhou 310016, Peoples R China

[5] Chinese Acad Sci, Kunming Inst Zool, KIZ CUHK Joint Lab Bioresources & Mol Res Common, Kunming 650223, Yunnan, Peoples R China

[6] Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China

来源：

JOURNAL OF MOLECULAR CELL BIOLOGY | 2020年 / 12卷 / 11期

基金：

中国国家自然科学基金;

关键词：

imprinted gene; imprinted gene network; mammary gland; stem cell; differentiation; metabolism; GENE NETWORK; BREAST-CANCER; EXPRESSION; GROWTH; PACKAGE; PROTEIN; CELLS; RNAS;

D O I：

10.1093/jmcb/mjaa020

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Genomic imprinting is an epigenetic modification of DNA, whereby gene expression is restricted to either maternally or paternally inherited alleles. Imprinted genes (IGs) in the placenta and embryo are essential for growth regulation and nutrient supply. However, despite being an important nutrition delivery organ, studies on mammary gland genomic imprinting remain limited. In this study, we found that both the number of IGs and their expression levels decreased during development of the mouse mammary gland. IG expression was lineage-specific and related to mammary gland development and lactation. Meta-analysis of single-cell RNA sequencing data revealed that mammary gland IGs were co-expressed in a network that regulated cell stemness and differentiation, which was confirmed by our functional studies. Accordingly, our data indicated that IGs were essential for the self-renewal of mammary gland stem cells and IG decline was correlated with mammary gland maturity. Taken together, our findings revealed the importance of IGs in a poorly studied nutrition-related organ, i.e. the mammary gland, thus providing a reference for further studies on genomic imprinting.

引用

页码：857 / 869

页数：13

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