Microarray gene expression profiling provides insights into functions of TIPE2 in HBV-related apoptosis

被引：2

作者：

Cui, Jian ^{[1
,2
]}

Zhang, Na ^{[1
]}

Liu, Ying ^{[1
]}

Zhang, Lei ^{[1
]}

Gao, Chengjiang ^{[1
,2
]}

Liu, Suxia ^{[1
,2
]}

机构：

[1] Shandong Univ, Sch Basic Med Sci, Dept Immunol, 44 Wenhua West Rd, Jinan 250012, Peoples R China

[2] Shandong Univ, Sch Basic Med Sci, Key Lab Infect & Immun Shandong Prov, Jinan, Peoples R China

来源：

MOLECULAR IMMUNOLOGY | 2021年 / 131卷

基金：

中国国家自然科学基金;

关键词：

TNFAIP8L2 (TIPE2); Microarray gene expression; Hepatitis B; Apoptosis; Inflammation;

D O I：

10.1016/j.molimm.2020.12.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tumor necrosis factor-a-induced protein-8 like-2 (TNFAIP8L2, TIPE2), a member of TNFAIP8 family, functions as a regulator in inflammation. Our previous studies showed that TIPE2 can negatively regulate HBV-specific CD8+ T lymphocyte functions. But the effect of TIPE2 on the apoptosis of HBV-infected hepatocytes which is very important for eliminating viruses remains unclear. Using gene expression microarray analysis, we find that TIPE2 deficiency can regulate the expression of apoptotic genes in liver tissues from HBV hydrodynamic injection (HI) mouse model. TIPE2 protein was detected in TUNEL staining positive hepatocytes in HBV-infected C57 mice. Interestingly, the TIPE2 expressed hepatocytes were just the HBV infected cells. Furthermore, TIPE2 upregulates the mRNA levels of FasL, Bim and TNFRsF1b which promote cells death, when TIPE2 was transfected into HepG2 cells in vitro. As a result, TIPE2 overexpression cells showed a higher number of apoptotic cells and increased level of cleavage caspase3 compared to controls. Those results indicate that TIPE2 participates in HBV infection by regulating apoptosis of virus-infected hepatocytes.

引用

页码：137 / 143

页数：7