A.m. is a world-wide pathogen for conifers, ornamental trees as well as fruit trees such as Prunica persica. Cytochromes e oxidase, c and b were detectable in mitochondria isolated from A.m. rhizomorphe grown in semi-solid malt-agar-sucrose medium. The mitochondria oxidized NADH, succinate and TMPD-ascorbate; rotenone, antimycin A, and KCN inhibited NADH, succinate and TMPD-ascorbate oxidation, respectively. In mitochondria isolated from rhizomorphe grown in liquid medium with glucose, cytochrome c oxidase was not detectable, while cytochromes b and c were present. In mitochondria isolated from A.m. rhizomorphe grown in liquid medium With ethanol, or from A.m. mushroom (carpophore, cap and stem), all three cytochromes were present. Data showed that A.m. in the mushroom state or cultivated in vitro exhibited all cytochromes, but that it lacked cytochrome c oxidase when cultivated with glucose. suggesting an alternative electron transport path,,way in its mitochondria.
