TNF-α detection using gold nanoparticles as a surface-enhanced Raman spectroscopy substrate

被引：3
|
作者：
Loredo-Garcia, Elizabeth [1 ]
Ortiz-Dosal, Alejandra [2 ]
Manuel Nunez-Leyva, Juan [2 ]
Cuellar Camacho, Jose Luis [3 ]
Alejandro Alegria-Torres, Jorge [4 ]
Garcia-Torres, Lizeth [5 ]
Ricardo Navarro-Contreras, Hugo [1 ]
Samuel Kolosovas-Machuca, Eleazar [1 ]
机构：
[1] Univ Autonoma San Luis Potosi, Coordinac Innovac & Aplicac Ciencia & Tecnol, 550 Sierra Leona Av, San Luis Potosi 78210, San Luis Potosi, Mexico
[2] Univ Autonoma San Luis Potosi, Ingn & Ciencia Mat DICIM UASLP, 550 Sierra Leona Av, San Luis Potosi 78210, San Luis Potosi, Mexico
[3] Free Univ Berlin, Inst Chem & Biochem, Takustr 3, D-14195 Berlin, Germany
[4] Univ Guanajuato, Dept Farm, Div Ciencias Nat & Exactas, Noria Alta S-N, Guanajuato 36050, Gto, Mexico
[5] Univ Ctr Mexico, Lab Invest Mol Nutr LIMON, UCEM, Capitan Caldera 75, San Luis Potosi 78250, San Luis Potosi, Mexico
来源：
NANOMEDICINE | 2020年 / 16卷 / 01期
关键词：
biomarkers; cytokines; gold nanoparticles; SERS; TNF-α TUMOR-NECROSIS-FACTOR; AMINO-ACIDS; OPTICAL-PROPERTIES; SERS;
D O I：
10.2217/nnm-2020-0307
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background: TNF-alpha is a cytokine involved in inflammation. Surface-enhanced Raman spectroscopy (SERS) could be useful in its detection. Aim: Identify the TNF-alpha in an aqueous solution, using gold nanoparticles (AuNPs) as a SERS substrate. Materials & methods: Raman and SERS spectra were obtained from TNF-alpha samples, combined with AuNPs, with decreasing concentrations of TNF-alpha. The samples were analyzed using optical transmission spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: Transmission electron microscopy/dynamic light scattering determined a change in the average diameter of the TNF-alpha/AuNPs (similar to 9.6 nm). Raman bands obtained were associated with aromatic amino acid side chains. We observe Raman signals for TNF-alpha concentrations as low as 0.125 pg/ml. Conclusion: TNF-alpha signal at physiological concentrations was determined with SERS.
引用
收藏
页码：51 / 61
页数：11
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