TNF-α detection using gold nanoparticles as a surface-enhanced Raman spectroscopy substrate

被引：3

作者：

Loredo-Garcia, Elizabeth ^{[1
]}

Ortiz-Dosal, Alejandra ^{[2
]}

Manuel Nunez-Leyva, Juan ^{[2
]}

Cuellar Camacho, Jose Luis ^{[3
]}

Alejandro Alegria-Torres, Jorge ^{[4
]}

Garcia-Torres, Lizeth ^{[5
]}

Ricardo Navarro-Contreras, Hugo ^{[1
]}

Samuel Kolosovas-Machuca, Eleazar ^{[1
]}

机构：

[1] Univ Autonoma San Luis Potosi, Coordinac Innovac & Aplicac Ciencia & Tecnol, 550 Sierra Leona Av, San Luis Potosi 78210, San Luis Potosi, Mexico

[2] Univ Autonoma San Luis Potosi, Ingn & Ciencia Mat DICIM UASLP, 550 Sierra Leona Av, San Luis Potosi 78210, San Luis Potosi, Mexico

[3] Free Univ Berlin, Inst Chem & Biochem, Takustr 3, D-14195 Berlin, Germany

[4] Univ Guanajuato, Dept Farm, Div Ciencias Nat & Exactas, Noria Alta S-N, Guanajuato 36050, Gto, Mexico

[5] Univ Ctr Mexico, Lab Invest Mol Nutr LIMON, UCEM, Capitan Caldera 75, San Luis Potosi 78250, San Luis Potosi, Mexico

来源：

NANOMEDICINE | 2020年 / 16卷 / 01期

关键词：

biomarkers; cytokines; gold nanoparticles; SERS; TNF-α TUMOR-NECROSIS-FACTOR; AMINO-ACIDS; OPTICAL-PROPERTIES; SERS;

D O I：

10.2217/nnm-2020-0307

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: TNF-alpha is a cytokine involved in inflammation. Surface-enhanced Raman spectroscopy (SERS) could be useful in its detection. Aim: Identify the TNF-alpha in an aqueous solution, using gold nanoparticles (AuNPs) as a SERS substrate. Materials & methods: Raman and SERS spectra were obtained from TNF-alpha samples, combined with AuNPs, with decreasing concentrations of TNF-alpha. The samples were analyzed using optical transmission spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: Transmission electron microscopy/dynamic light scattering determined a change in the average diameter of the TNF-alpha/AuNPs (similar to 9.6 nm). Raman bands obtained were associated with aromatic amino acid side chains. We observe Raman signals for TNF-alpha concentrations as low as 0.125 pg/ml. Conclusion: TNF-alpha signal at physiological concentrations was determined with SERS.

引用

页码：51 / 61

页数：11

共 37 条

[31] Surface enhanced Raman scattering with gold nanoparticles: effect of particle shape [J].

Tian, Furong ;

Bonnier, Franck ;

Casey, Alan ;

Shanahan, Anne E. ;

Byrne, Hugh J. .

ANALYTICAL METHODS, 2014, 6 (22) :9116-9123

[32] Diagnostic methods for the measurement of human TNF-alpha in clinical laboratory [J].

Valaperti, Alan ;

Li, Zhe ;

Vonow-Eisenring, Maya ;

Probst-Muller, Elisabeth .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2020, 179

[33] Tumor necrosis factor signaling [J].

Wajant, H ;

Pfizenmaier, K ;

Scheurich, P .

CELL DEATH AND DIFFERENTIATION, 2003, 10 (01) :45-65

[34] Surface-Enhanced Raman Spectroscopy-Based, Homogeneous, Multiplexed Immunoassay with Antibody-Fragments-Decorated Gold Nanoparticles [J].

Wang, Yu ;

Tang, Li-Juan ;

Jiang, Jian-Hui .

ANALYTICAL CHEMISTRY, 2013, 85 (19) :9213-9220

[35] Aromatic amino acids providing characteristic motifs in the Raman and SERS spectroscopy of peptides [J].

Wei, Fang ;

Zhang, Dongmao ;

Halas, Naomi J. ;

Hartgerink, Jeffrey D. .

JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (30) :9158-9164

[36] Label-free SERS in biological and biomedical applications: Recent progress, current challenges and opportunities [J].

Zheng, Xiao-Shan ;

Jahn, Izabella Jolan ;

Weber, Karina ;

Cialla-May, Dana ;

Popp, Juergen .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2018, 197 :56-77

[37]

Zhu GY, 2011, SPECTROCHIM ACTA A, V78, P1187, DOI [10.1016/j.saa.2010.12.079, 10.1016/j.saa.2010.12.07]

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