TNF-α detection using gold nanoparticles as a surface-enhanced Raman spectroscopy substrate

被引：3

作者：

Loredo-Garcia, Elizabeth ^{[1
]}

Ortiz-Dosal, Alejandra ^{[2
]}

Manuel Nunez-Leyva, Juan ^{[2
]}

Cuellar Camacho, Jose Luis ^{[3
]}

Alejandro Alegria-Torres, Jorge ^{[4
]}

Garcia-Torres, Lizeth ^{[5
]}

Ricardo Navarro-Contreras, Hugo ^{[1
]}

Samuel Kolosovas-Machuca, Eleazar ^{[1
]}

机构：

[1] Univ Autonoma San Luis Potosi, Coordinac Innovac & Aplicac Ciencia & Tecnol, 550 Sierra Leona Av, San Luis Potosi 78210, San Luis Potosi, Mexico

[2] Univ Autonoma San Luis Potosi, Ingn & Ciencia Mat DICIM UASLP, 550 Sierra Leona Av, San Luis Potosi 78210, San Luis Potosi, Mexico

[3] Free Univ Berlin, Inst Chem & Biochem, Takustr 3, D-14195 Berlin, Germany

[4] Univ Guanajuato, Dept Farm, Div Ciencias Nat & Exactas, Noria Alta S-N, Guanajuato 36050, Gto, Mexico

[5] Univ Ctr Mexico, Lab Invest Mol Nutr LIMON, UCEM, Capitan Caldera 75, San Luis Potosi 78250, San Luis Potosi, Mexico

来源：

NANOMEDICINE | 2020年 / 16卷 / 01期

关键词：

biomarkers; cytokines; gold nanoparticles; SERS; TNF-α TUMOR-NECROSIS-FACTOR; AMINO-ACIDS; OPTICAL-PROPERTIES; SERS;

D O I：

10.2217/nnm-2020-0307

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: TNF-alpha is a cytokine involved in inflammation. Surface-enhanced Raman spectroscopy (SERS) could be useful in its detection. Aim: Identify the TNF-alpha in an aqueous solution, using gold nanoparticles (AuNPs) as a SERS substrate. Materials & methods: Raman and SERS spectra were obtained from TNF-alpha samples, combined with AuNPs, with decreasing concentrations of TNF-alpha. The samples were analyzed using optical transmission spectroscopy, dynamic light scattering, and transmission electron microscopy. Results: Transmission electron microscopy/dynamic light scattering determined a change in the average diameter of the TNF-alpha/AuNPs (similar to 9.6 nm). Raman bands obtained were associated with aromatic amino acid side chains. We observe Raman signals for TNF-alpha concentrations as low as 0.125 pg/ml. Conclusion: TNF-alpha signal at physiological concentrations was determined with SERS.

引用

页码：51 / 61

页数：11

共 37 条

[1] Detection of hydroquinone by Raman spectroscopy in patients with melasma before and after treatment [J].

Cabrera-Alonso, R. ;

Guevara, E. ;

Ramirez-Elias, M. G. ;

Moncada, B. ;

Gonzalez, F. J. .

SKIN RESEARCH AND TECHNOLOGY, 2019, 25 (01) :20-24

[2] Dual-reporter SERS-based biomolecular assay with reduced false-positive signals [J].

Chuong, Tracy T. ;

Pallaoro, Alessia ;

Chaves, Chelsea A. ;

Li, Zhe ;

Lee, Joun ;

Eisenstein, Michael ;

Stucky, Galen D. ;

Moskovits, Martin ;

Soh, H. Tom .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (34) :9056-9061

[3] The many faces of tumor necrosis factor signaling in the intestinal epithelium [J].

Delgado, M. Eugenia ;

Brunner, Thomas .

GENES AND IMMUNITY, 2019, 20 (08) :609-626

[4] Historical insights into cytokines [J].

Dinarello, Charles A. .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2007, 37 :S34-S45

[5] Technical note: Validation of an ELISA for measurement of tumor necrosis factor alpha in bovine plasma [J].

Farney, J. K. ;

Mamedova, L. K. ;

Godsey, B. H. ;

Bradford, B. J. .

JOURNAL OF DAIRY SCIENCE, 2011, 94 (07) :3504-3509

[6] SERS Quantification and Characterization of Proteins and Other Biomolecules [J].

Feliu, Neus ;

Hassan, Moustapha ;

Garcia Rico, Eduardo ;

Cui, Daxiang ;

Parak, Wolfgang ;

Alvarez-Puebla, Ramon .

LANGMUIR, 2017, 33 (38) :9711-9730

[7] Effect of gold nanoparticle structure on the conformation and function of adsorbed proteins [J].

Gagner, Jennifer E. ;

Qian, Xi ;

Lopez, Maria M. ;

Dordick, Jonathan S. ;

Siegel, Richard W. .

BIOMATERIALS, 2012, 33 (33) :8503-8516

[8] Effect of gold nanoparticle morphology on adsorbed protein structure and function [J].

Gagner, Jennifer E. ;

Lopez, Marimar D. ;

Dordick, Jonathan S. ;

Siegel, Richard W. .

BIOMATERIALS, 2011, 32 (29) :7241-7252

[9] SURFACE-ENHANCED RAMAN-SPECTROSCOPIC INVESTIGATION OF HUMAN IMMUNOGLOBULIN-G ADSORBED ON A SILVER ELECTRODE [J].

GRABBE, ES ;

BUCK, RP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (22) :8362-8366

[10] SERS:: a versatile tool in chemical and biochemical diagnostics [J].

Hering, Katharina ;

Cialla, Dana ;

Ackermann, Katrin ;

Doerfer, Thomas ;

Moeller, Robert ;

Schneidewind, Henrik ;

Mattheis, Roland ;

Fritzsche, Wolfgang ;

Roesch, Petra ;

Popp, Juergen .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 390 (01) :113-124

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