Induction of heme oxygenase 1 by arsenite inhibits cytokine-induced monocyte adhesion to human endothelial cells

被引：14

作者：

Sun, Xi ^{[1
]}

Pi, Jingbo ^{[2
]}

Liu, Wenlan ^{[1
]}

Hudson, Laurie G. ^{[1
]}

Liu, Ke Jian ^{[1
]}

Feng, Changjian ^{[1
]}

机构：

[1] Univ New Mexico 1, Coll Pharm, Albuquerque, NM 87131 USA

[2] Hamner Inst Hlth Sci, Div Translat Biol, Res Triangle Pk, NC 27709 USA

来源：

TOXICOLOGY AND APPLIED PHARMACOLOGY | 2009年 / 236卷 / 02期

关键词：

Arsenic; Endothelial cells; Heme oxygenase 1; Cytokine; Monocyte adhesion; NF-KAPPA-B; GENE-EXPRESSION; CARDIOVASCULAR-DISEASE; DRINKING-WATER; CHEMOATTRACTANT PROTEIN-1; VASCULAR-DISEASE; OXIDATIVE STRESS; IN-VITRO; EXPOSURE; ATHEROSCLEROSIS;

D O I：

10.1016/j.taap.2009.01.023

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Heme oxygenase-1 (HO-1) is an oxidative stress responsive gene upregulated by various physiological and exogenous stimuli. Arsenite, as an oxidative stressor, is a potent inducer of HO-1 in human and rodent cells. In this study, we investigated the mechanistic role of arsenite-induced HO-1 in modulating tumor necrosis Factor alpha (TNF-alpha) induced monocyte adhesion to human umbilical vein endothelial cells (HUVEC). Arsenite pretreatment, which upregulated HO-1 in a time and concentration-dependent manner, inhibited TNF-alpha-induced monocyte adhesion to HUVEC and intercellular adhesion molecule 1 protein expression by 50% and 40%, respectively importantly, knockdown of HO-1 by small interfering RNA abolished the arsenite-induced inhibitory effects. These results indicate that induction of HO-1 by arsenite inhibits the cytokine-induced monocyte adhesion to HUVEC by suppressing adhesion molecule expression. These Findings established in important mechanistic link between the functional monocyte adhesion properties of HUVEC and the induction of HO-1 by arsenite. (C) 2009 Elsevier Inc. All rights reserved

引用

页码：202 / 209

页数：8

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