Characterization and mapping of simple sequence repeats (SSRs) in Sorghum bicolor

被引：114
作者：
Taramino, G
Tarchini, R
Ferrario, S
Lee, M
Pe, ME
机构：
[1] UNIV MILAN, DIPARTIMENTO GENET & BIOL MICRORGANISMI, I-20133 MILAN, ITALY
[2] IOWA STATE UNIV, DEPT AGRON, AMES, IA 50011 USA
来源：
THEORETICAL AND APPLIED GENETICS | 1997年 / 95卷 / 1-2期
关键词：
Sorghum bicolor; simple sequence repeats (SSRs); polymorphism; mapping;
D O I：
10.1007/s001220050533
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
A total of 13 SSR loci were characterized in Sorghum bicolor. Ten of these loci were isolated by screening sorghum genomic AG-enriched libraries with labelled poly(AC)/poly(CT), the other three were derived from database searches. In order to explore the degree of polymorphism detectable in this species by this type of molecular marker: the SSR markers were tested on nine inbred lines of S. bicolor of different geographic origin. PCR analysis on acrylamide gels revealed a high degree of polymorphism (delta(T) = 0.80), One locus, in particular, allowed the identification of all of the nine inbred lines used in our study. Seven of these SSR markers were mapped, using an existing sorghum RFLP map.
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页码：66 / 72
页数：7
相关论文
共 37 条
[1]   PATTERNS OF ALLOZYME VARIATION IN CULTIVATED AND WILD SORGHUM-BICOLOR [J].
ALDRICH, PR ;
DOEBLEY, J ;
SCHERTZ, KF ;
STEC, A .
THEORETICAL AND APPLIED GENETICS, 1992, 85 (04) :451-460
[2]   RESTRICTION FRAGMENT VARIATION IN THE NUCLEAR AND CHLOROPLAST GENOMES OF CULTIVATED AND WILD SORGHUM-BICOLOR [J].
ALDRICH, PR ;
DOEBLEY, J .
THEORETICAL AND APPLIED GENETICS, 1992, 85 (2-3) :293-302
[3]  
[Anonymous], 1988, ECON BOT
[4]   SIMILARITY OF MAIZE AND SORGHUM GENOMES AS REVEALED BY MAIZE RFLP PROBES [J].
BINELLI, G ;
GIANFRANCESCHI, L ;
PE, ME ;
TARAMINO, G ;
BUSSO, C ;
STENHOUSE, J ;
OTTAVIANO, E .
THEORETICAL AND APPLIED GENETICS, 1992, 84 (1-2) :10-16
[5]  
Broun P, 1996, MOL GEN GENET, V250, P39, DOI 10.1007/s004380050049
[6]   A DETAILED RFLP MAP OF SORGHUM-BICOLOR X S-PROPINQUUM, SUITABLE FOR HIGH-DENSITY MAPPING, SUGGESTS ANCESTRAL DUPLICATION OF SORGHUM CHROMOSOMES OR CHROMOSOMAL SEGMENTS [J].
CHITTENDEN, LM ;
SCHERTZ, KF ;
LIN, YR ;
WING, RA ;
PATERSON, AH .
THEORETICAL AND APPLIED GENETICS, 1994, 87 (08) :925-933
[7]  
Cregan Perry B., 1994, Methods in Molecular and Cellular Biology, V5, P49
[8]   RFLP-BASED ASSAY OF SORGHUM-BICOLOR (L) MOENCH GENETIC DIVERSITY [J].
CUI, YX ;
XU, GW ;
MAGILL, CW ;
SCHERTZ, KF ;
HART, GE .
THEORETICAL AND APPLIED GENETICS, 1995, 90 (06) :787-796
[9]  
DELLAPORTA SL, 1985, MOL BIOL PLANTS
[10]   CHARACTERIZATION OF THE KAFIRIN GENE FAMILY FROM SORGHUM REVEALS EXTENSIVE HOMOLOGY WITH ZEIN FROM MAIZE [J].
DEROSE, RT ;
MA, DP ;
KWON, IS ;
HASNAIN, SE ;
KLASSY, RC ;
HALL, TC .
PLANT MOLECULAR BIOLOGY, 1989, 12 (03) :245-256
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