Induction of apoptosis by opium in some tumor cell lines

被引：1

作者：

Khaleghi, M. ^{[1
]}

Farsinejad, A. ^{[2
]}

Dabiri, S. ^{[2
]}

Asadikaram, G. ^{[3
,4
]}

机构：

[1] Kerman Univ Med Sci, Inst Neuropharmacol, Neurosci Res Ctr, Kerman, Iran

[2] Kerman Univ Med Sci, Pathol & Stem Cell Res Ctr, Kerman, Iran

[3] Kerman Univ Med Sci, Sch Med, Inst Basic & Clin Physiol Sci, Endocrinol & Metab Res Ctr, Kerman, Iran

[4] Kerman Univ Med Sci, Sch Med, Dept Biochem, Kerman, Iran

来源：

CELLULAR AND MOLECULAR BIOLOGY | 2016年 / 62卷 / 11期

关键词：

Opium; Apoptosis; Necrosis; Cell lines; Flow-cytometry; IN-VITRO; MORPHINE; NEURONS; PATHWAY; CODEINE; VIRUS; DEATH;

D O I：

10.14715/cmb/2016.62.11.13

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The current study is aimed at investigation of the opium effects on the apoptosis of different cell lines in culture medium and compares such effects with one another. The study is carried out on over 8 cell lines (AA8, AGS, Hela, HepG2, MCF7, N2a, PC12, WEHI). A 2.86 x 10(-4) g/ml opium concentration was prepared and added to the culture medium of the cell lines for 48 hours. Cytotoxicity was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptotic effect of opium on the cell lines was analyzed by Annexin-PI test. Opium with concentration of 2.86 x 10(-4) g/ml in 48 hours significantly induces apoptosis in certain cell lines (i.e. AA8, N2a, WEHI), apoptosis and necrosis in some others (i. e. Hela, HepG2, MCF7, and PC12), and also solely necrosis in the AGS cell line. One could infer that the usage of opium with different levels in different tissues leads to certain disorders in some tissues and may have therapeutic effects under distinctive conditions (i.e. unchecked growth of cells) as confirmed by the results.

引用

页码：76 / 80

页数：5